[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

[HTML][HTML] An introduction to nanopore sequencing: past, present, and future considerations

M MacKenzie, C Argyropoulos - Micromachines, 2023 - mdpi.com
There has been significant progress made in the field of nanopore biosensor development
and sequencing applications, which address previous limitations that restricted widespread …

[HTML][HTML] Oxford Nanopore R10. 4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short …

M Sereika, RH Kirkegaard, SM Karst, TY Michaelsen… - Nature …, 2022 - nature.com
Abstract Long-read Oxford Nanopore sequencing has democratized microbial genome
sequencing and enables the recovery of highly contiguous microbial genomes from isolates …

[HTML][HTML] Advances in nanopore direct RNA sequencing

M Jain, R Abu-Shumays, HE Olsen, M Akeson - Nature methods, 2022 - nature.com
Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early
adopters have used this technology to document nucleotide modifications and 3 …

[HTML][HTML] Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic

M Vandenbogaert, A Kwasiborski, E Gonofio… - Scientific reports, 2022 - nature.com
Monkeypox is an emerging and neglected zoonotic disease whose number of reported
cases has been gradually increasing in Central Africa since 1980. This disease is caused by …

The newest Oxford Nanopore R10. 4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling

T Zhang, H Li, S Ma, J Cao, H Liao… - Applied and …, 2023 - Am Soc Microbiol
The long-read amplicon provides a species-level solution for the community. With the
improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) …

[HTML][HTML] Comparative evaluation of Nanopore polishing tools for microbial genome assembly and polishing strategies for downstream analysis

JY Lee, M Kong, J Oh, JS Lim, SH Chung, JM Kim… - Scientific Reports, 2021 - nature.com
Assembling high-quality microbial genomes using only cost-effective Nanopore long-read
systems such as Flongle is important to accelerate research on the microbial genome and …

[HTML][HTML] From molecules to genomic variations: Accelerating genome analysis via intelligent algorithms and architectures

M Alser, J Lindegger, C Firtina, N Almadhoun… - Computational and …, 2022 - Elsevier
We now need more than ever to make genome analysis more intelligent. We need to read,
analyze, and interpret our genomes not only quickly, but also accurately and efficiently …

Unveiling microbial diversity: harnessing long-read sequencing technology

DP Agustinho, Y Fu, VK Menon, GA Metcalf… - Nature …, 2024 - nature.com
Long-read sequencing has recently transformed metagenomics, enhancing strain-level
pathogen characterization, enabling accurate and complete metagenome-assembled …

[HTML][HTML] Modification mapping by nanopore sequencing

LK White, JR Hesselberth - Frontiers in Genetics, 2022 - frontiersin.org
Next generation sequencing (NGS) has provided biologists with an unprecedented view into
biological processes and their regulation over the past 2 decades, fueling a wave of …