Human pluripotent stem cells (hPSCs) provide powerful resources for application in
regenerative medicine and pharmaceutical development. In the past decade, various …

Immune rejection is an important obstacle to the use of allogeneic 'off-the-shelf'cells for
transplantation into immunocompetent patients. Genetic modification has emerged as a …

Precise gene editing in human pluripotent stem cells (hPSCs) holds great promise for
studying and potentially treating human diseases. Both prime editing and base editing avoid …

Silencing and variegated transgene expression are poorly understood problems that can
interfere with gene function studies in human embryonic stem cells (hESCs). We show that …

Background Constitutive promoters that ensure sustained and high level gene expression
are basic research tools that have a wide range of applications, including studies of human …

Culture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications
in research and clinic. However, the mechanisms that underpin selection of variants remain …

Transgenesis is a cornerstone of molecular biology. The ability to integrate a specifically
engineered piece of DNA into the genome of a living system is fundamental to our efforts to …

Regulatable transgene expression in human pluripotent stem cells (hPSCs) and their
progenies is often necessary to dissect gene function in a temporal and spatial manner …

The appearance of genetic changes in human pluripotent stem cells (hPSCs) presents a
concern for their use in research and regenerative medicine. Variant hPSCs that harbor …

Cancer stem cells (CSCs) are associated with tumor progression, recurrence, and
therapeutic resistance. To maintain their pool while promoting tumorigenesis, CSCs divide …