East African cichlid fishes

ME Santos, JF Lopes, CF Kratochwil - EvoDevo, 2023 - Springer
Cichlid fishes are a very diverse and species-rich family of teleost fishes that inhabit lakes
and rivers of India, Africa, and South and Central America. Research has largely focused on …

Myoglobin in Brown Adipose Tissue: A Multifaceted Player in Thermogenesis

MA Aboouf, TA Gorr, NM Hamdy, M Gassmann… - Cells, 2023 - mdpi.com
Brown adipose tissue (BAT) plays an important role in energy homeostasis by generating
heat from chemical energy via uncoupled oxidative phosphorylation. Besides its high …

Rapid and precise genome engineering in a naturally short-lived vertebrate

CN Bedbrook, RD Nath, R Nagvekar, K Deisseroth… - Elife, 2023 - elifesciences.org
The African turquoise killifish is a powerful vertebrate system to study complex phenotypes
at scale, including aging and age-related disease. Here, we develop a rapid and precise …

Modular control of vertebrate axis segmentation in time and space

A Seleit, I Brettell, T Fitzgerald, C Vibe, F Loosli… - The EMBO …, 2024 - embopress.org
How the timing of development is linked to organismal size is a longstanding question.
Although numerous studies have reported a correlation of temporal and spatial traits, the …

Endogenous tagging of multiple cellular components in the sea anemone Nematostella vectensis

A Paix, S Basu, P Steenbergen… - Proceedings of the …, 2023 - National Acad Sciences
The cnidarian Nematostella vectensis has developed into a powerful model system to study
the mechanisms underlying animal development, regeneration, and evolution. However …

Knock-in tagging in zebrafish facilitated by insertion into non-coding regions

DS Levic, N Yamaguchi, S Wang, H Knaut… - …, 2021 - journals.biologists.com
Zebrafish provide an excellent model for in vivo cell biology studies because of their
amenability to live imaging. Protein visualization in zebrafish has traditionally relied on …

Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata

JF Warner, R Besemer, A Schickle, E Borbee… - …, 2023 - pmc.ncbi.nlm.nih.gov
Cnidarians have become valuable models for understanding many aspects of
developmental biology including the evolution of body plan diversity, novel cell type …

Efficient knock-in method enabling lineage tracing in zebrafish

J Mi, O Andersson - Life Science Alliance, 2023 - life-science-alliance.org
Here, we devised a cloning-free 3′ knock-in strategy for zebrafish using PCR amplified
dsDNA donors that avoids disrupting the targeted genes. The dsDNA donors carry genetic …

Super-Resolution Microscopy for Protein Imaging: Unraveling Cellular Architecture and Function

N Rabiee - TrAC Trends in Analytical Chemistry, 2025 - Elsevier
Super-resolution microscopy has emerged as a groundbreaking technique in cell biology,
enabling researchers to visualize proteins and their interactions with unprecedented spatial …

Genome editing in East African cichlids and tilapias: state-of-the-art and future directions

B Clark, M Kuwalekar, B Fischer… - Open …, 2023 - royalsocietypublishing.org
African cichlid fishes of the Cichlidae family are a group of teleosts important for aquaculture
and research. A thriving research community is particularly interested in the cichlid …