N Fukuda, H Tanaka, Y Shoyama - Journal of Natural Products, 2000 - ACS Publications
Immunoaffinity column chromatography using an anti-ginsenoside Rb1 monoclonal antibody has made possible a single-step separation of ginsenoside Rb1 from a crude …
T Uto, T Ohta, S Fujii, Y Shoyama - Antibodies, 2021 - mdpi.com
In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its …
R Kumar, MI Khan, M Prasad - Research Journal of Pharmacy …, 2019 - indianjournals.com
Steroidal glycoalkaloids, a category of glycosidic ingradients are originated from nitrogen- containing steroids. Structurally it includes of C27 cholestane skeleton to that 1 to 5 sugar …
W Putalun, F Taura, W Qing, H Matsushita, H Tanaka… - Plant cell reports, 2003 - Springer
We constructed a recombinant antibody fragment—single chain fragment-variable (scFv) antibody—derived from hybridoma cell lines to control the concentration of solasodine …
J Xu, H Tanaka, Y Shoyama - Journal of Chromatography B, 2007 - Elsevier
The bioactive constituent, glycyrrhizin or glycyrrhizic acid (GA), was purified from two traditional Chinese medicines (TCM), Shaoyao gancao tang and Dahuang gancao tang …
O Morinaga, S Nakajima, H Tanaka, Y Shoyama - Analyst, 2001 - pubs.rsc.org
For immunization, sennoside B was conjugated with bovine serum albumin. The hapten density in the antigen conjugate was determined to be 3 mol mol− 1 protein by matrix …
Miroestrol is a chromene with potent estrogenic activity present in Pueraria candollei, commonly known as White Kwao Krua. Although this compound is only present in low …
H Tanaka, N Fukuda, Y Shoyama - Journal of agricultural and food …, 2007 - ACS Publications
Ginsenosides separated by silica gel TLC blotted to a PVDF membrane that was treated with a NaIO4 solution followed by bovine serum albumin (BSA) resulted in a ginsenoside− BSA …
H Qu, Y Zhang, B Qu, J Cheng, S Liu… - Journal of separation …, 2016 - Wiley Online Library
In this work, a novel monoclonal antibody specific for naringin was prepared and characterized. Subsequently, an indirect competitive enzyme‐linked immunosorbent assay …