Monoclonal antibodies and immunoassay for medical plant-derived natural products: a review

X Yan, Y Zhao, Y Zhang, H Qu - Molecules, 2017 - mdpi.com
Owing to the widespread application value, monoclonal antibodies (MAbs) have become a
tool of increasing importance in modern bioscience research since their emergence …

Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography

N Fukuda, H Tanaka, Y Shoyama - Journal of Natural Products, 2000 - ACS Publications
Immunoaffinity column chromatography using an anti-ginsenoside Rb1 monoclonal
antibody has made possible a single-step separation of ginsenoside Rb1 from a crude …

Immunological separation of bioactive natural compounds from crude drug extract and its application for cell-based studies

T Uto, T Ohta, S Fujii, Y Shoyama - Antibodies, 2021 - mdpi.com
In this study, we present a review on a useful approach, namely, immunoaffinity column
coupled with monoclonal antibodies (MAbs), to separate natural compounds and its …

Solasodine: A Perspective on their roles in Health and Disease

R Kumar, MI Khan, M Prasad - Research Journal of Pharmacy …, 2019 - indianjournals.com
Steroidal glycoalkaloids, a category of glycosidic ingradients are originated from nitrogen-
containing steroids. Structurally it includes of C27 cholestane skeleton to that 1 to 5 sugar …

Anti-solasodine glycoside single-chain Fv antibody stimulates biosynthesis of solasodine glycoside in plants

W Putalun, F Taura, W Qing, H Matsushita, H Tanaka… - Plant cell reports, 2003 - Springer
We constructed a recombinant antibody fragment—single chain fragment-variable (scFv)
antibody—derived from hybridoma cell lines to control the concentration of solasodine …

One-step immunochromatographic separation and ELISA quantification of glycyrrhizin from traditional Chinese medicines

J Xu, H Tanaka, Y Shoyama - Journal of Chromatography B, 2007 - Elsevier
The bioactive constituent, glycyrrhizin or glycyrrhizic acid (GA), was purified from two
traditional Chinese medicines (TCM), Shaoyao gancao tang and Dahuang gancao tang …

Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA

O Morinaga, S Nakajima, H Tanaka, Y Shoyama - Analyst, 2001 - pubs.rsc.org
For immunization, sennoside B was conjugated with bovine serum albumin. The hapten
density in the antigen conjugate was determined to be 3 mol mol− 1 protein by matrix …

Development of an enzyme-linked immunosorbent assay for determination of miroestrol using an anti-miroestrol monoclonal antibody

T Kitisripanya, S Krittanai, O Udomsin, K Jutathis… - Planta …, 2017 - thieme-connect.com
Miroestrol is a chromene with potent estrogenic activity present in Pueraria candollei,
commonly known as White Kwao Krua. Although this compound is only present in low …

Eastern blotting and immunoaffinity concentration using monoclonal antibody for ginseng saponins in the field of traditional Chinese medicines

H Tanaka, N Fukuda, Y Shoyama - Journal of agricultural and food …, 2007 - ACS Publications
Ginsenosides separated by silica gel TLC blotted to a PVDF membrane that was treated with
a NaIO4 solution followed by bovine serum albumin (BSA) resulted in a ginsenoside− BSA …

Novel immunoassay and rapid immunoaffinity chromatography method for the detection and selective extraction of naringin in Citrus aurantium

H Qu, Y Zhang, B Qu, J Cheng, S Liu… - Journal of separation …, 2016 - Wiley Online Library
In this work, a novel monoclonal antibody specific for naringin was prepared and
characterized. Subsequently, an indirect competitive enzyme‐linked immunosorbent assay …