Innovation and new molecular entity production by the pharmaceutical industry has been
below expectations. Surprisingly, more first-in-class small-molecule drugs approved by the …

Introduction: In recent years, infections caused by multidrug-resistant bacterial pathogens
have become a huge issue to public healthcare systems. Indeed, the misuse of antibiotics …

The exploration of single cells reveals cell heterogeneity and biological principle of cellular
metabolism. Although a number of mass spectrometry (MS) based single cell MS (SCMS) …

Abstract Caveolin-1 (CAV1) is over-expressed in prostate cancer (PCa) and is associated
with adverse prognosis, but the molecular mechanisms linking CAV1 expression to disease …

Tissue Phenomics is the discipline of mining tissue images to identify patterns that are
related to clinical outcome providing potential prognostic and predictive value. This involves …

One of the greatest challenges in biomedical research, drug discovery and diagnostics is
understanding how seemingly identical cells can respond differently to perturbagens …

Modern flow cytometers can make optical measurements of 10 or more parameters per cell
at tens of thousands of cells per second and more than five orders of magnitude dynamic …

Rab8 is a small Ras-related GTPase that regulates polarized membrane transport to the
plasma membrane. Here, we developed a high-content analysis (HCA) tool to dissect Rab8 …

Background Cell-based assays comprising primary sensory neurons cultured in vitro are an
emerging tool for the screening and identification of potential analgesic compounds and …

This chapter discusses the image analysis component of tissue phenomics studies, together
with the pre-analytical variables that are critical for the successful execution of these studies …