Exploring the potential of cell-free protein synthesis for extending the abilities of biological systems

K Khambhati, G Bhattacharjee, N Gohil… - … in bioengineering and …, 2019 - frontiersin.org
Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …

Therapeutic alpha-interferons protein: structure, production, and biosimilar

NA El-Baky, EM Redwan - Preparative Biochemistry and …, 2015 - Taylor & Francis
In 2007, the world solemnized the golden jubilee of the discovery of interferon (IFN).
Interferon is a small protein messenger called a pluripotent cytokine, produced by several …

Effectiveness of human, camel, bovine and sheep lactoferrin on the hepatitis C virus cellular infectivity: comparison study

EM El-Fakharany, L Sánchez, HA Al-Mehdar… - Virology journal, 2013 - Springer
Purpose The prevalence of HCV infection has increased during recent years and the
incidence reach 3% of the world's population, and in some countries like Egypt, may around …

Influence of camel milk on the hepatitis C virus burden of infected patients

EM El-Fakharany, NA El-Baky… - Experimental and …, 2017 - spandidos-publications.com
Hepatitis C virus (HCV) infection represents a world health problem and no protective
vaccine or effective drug currently exists. For economic reasons, many patients use …

Screening the anti infectivity potentials of native N-and C-lobes derived from the camel lactoferrin against hepatitis C virus

EM Redwan, EM El-Fakharany, VN Uversky… - … and alternative medicine, 2014 - Springer
Abstract Background Hepatitis C virus (HCV) infection represents a worldwide health threat
that still needs efficient protective vaccine and/or effective drug. The traditional medicine …

Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7. 5 hepatoma

EM El-Fakharany, N Abedelbaky, BM Haroun… - Virology journal, 2012 - Springer
Purpose To extend the study of the camel milk proteins which have antiviral activity against
HCV, camel naïve polyclonal IgGs, α-lactalbumin were purified from camel milk and their …

Expression of soluble and active interferon consensus in SUMO fusion expression system in E. coli

K Peciak, R Tommasi, J Choi, S Brocchini… - Protein expression and …, 2014 - Elsevier
Protein production can be improved if methods for soluble protein expression are
developed. Interferon consensus (IFN-con) is used to treat hepatitis C. IFN-con has superior …

A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha

NA El-Baky, EM El-Fakharany, SA Sabry, ER El-Helow… - Biology, 2022 - mdpi.com
Simple Summary As a result of increasing demand for the pleiotropic cytokine TNF-α,
recombinant human TNF-α protein with appropriate bioactivities was produced in several …

[HTML][HTML] De novo expression and antibacterial potential of four lactoferricin peptides in cell-free protein synthesis system

N Abd El-Baky, MA Elkhawaga, ES Abdelkhalek… - Biotechnology …, 2021 - Elsevier
For the first time, we produced four lactoferricin (LFcin) peptides by a cell-free (in vitro)
method. These short antimicrobial peptides were expressed in an E. coli cell-free protein …

Auto-induction expression of human consensus interferon-alpha in Escherichia coli

NA EL-Baky, MH Linjawi, EM Redwan - BMC biotechnology, 2015 - Springer
Abstract Background Isopropyl-β-D-1-thiolgalactopyranoside (IPTG)-inducible expression of
recombinant proteins in E. coli is commonly used and effective. Nevertheless, unintended …