Cofilin and actin dynamics: multiple modes of regulation and their impacts in neuronal development and degeneration
JR Bamburg, LS Minamide, ON Wiggan… - Cells, 2021 - mdpi.com
Proteins of the actin depolymerizing factor (ADF)/cofilin family are ubiquitous among
eukaryotes and are essential regulators of actin dynamics and function. Mammalian neurons …
eukaryotes and are essential regulators of actin dynamics and function. Mammalian neurons …
Real-time single-molecule imaging of transcriptional regulatory networks in living cells
Gene regulatory networks drive the specific transcriptional programmes responsible for the
diversification of cell types during the development of multicellular organisms. Although our …
diversification of cell types during the development of multicellular organisms. Although our …
Xrn2 substrate mapping identifies torpedo loading sites and extensive premature termination of RNA pol II transcription
MA Cortazar, B Erickson, N Fong… - Genes & …, 2022 - genesdev.cshlp.org
The exonuclease torpedo Xrn2 loads onto nascent RNA 5′-PO 4 ends and chases down
pol II to promote termination downstream from polyA sites. We report that Xrn2 is recruited to …
pol II to promote termination downstream from polyA sites. We report that Xrn2 is recruited to …
RNA polymerase II clusters form in line with surface condensation on regulatory chromatin
It is essential for cells to control which genes are transcribed into RNA. In eukaryotes, two
major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and …
major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and …
[HTML][HTML] Single-cell stimulus-response gene expression trajectories reveal the stimulus specificities of dynamic responses by single macrophages
Macrophages induce the expression of hundreds of genes in response to immune threats.
However, current technology limits our ability to capture single-cell inducible gene …
However, current technology limits our ability to capture single-cell inducible gene …
In Situ Self‐Assembly of Fluorogenic RNA Nanozipper Enables Real‐Time Imaging of Single Viral mRNA Translation
YP Zhang, ZG Wang, YF Tian, LH Jiang… - Angewandte Chemie …, 2023 - Wiley Online Library
Real‐time visualization of individual viral mRNA translation activities in live cells is essential
to obtain critical details of viral mRNA dynamics and to detect its transient responses to …
to obtain critical details of viral mRNA dynamics and to detect its transient responses to …
Single-molecule tracking of transcription protein dynamics in living cells: seeing is believing, but what are we seeing?
Highlights•Transcription is controlled by large numbers of factors. Mechanistic models
require quantitative approaches.•Single Molecule Tracking provides direct measurements of …
require quantitative approaches.•Single Molecule Tracking provides direct measurements of …
Live imaging of transcription sites using an elongating RNA polymerase II–specific probe
In eukaryotic nuclei, most genes are transcribed by RNA polymerase II (RNAP2), whose
regulation is a key to understanding the genome and cell function. RNAP2 has a long …
regulation is a key to understanding the genome and cell function. RNAP2 has a long …
Live-cell imaging uncovers the relationship between histone acetylation, transcription initiation, and nucleosome mobility
Histone acetylation and RNA polymerase II phosphorylation are associated with
transcriptionally active chromatin, but their spatiotemporal relationship in live cells remains …
transcriptionally active chromatin, but their spatiotemporal relationship in live cells remains …
PIP2-Effector protein MPRIP regulates RNA polymerase II condensation and transcription
The specific post-translational modifications of the C-terminal domain (CTD) of the Rpb1
subunit of RNA polymerase II (RNAPII) correlate with different stages of transcription. The …
subunit of RNA polymerase II (RNAPII) correlate with different stages of transcription. The …