Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …

Directed evolution has revolutionized biomolecular engineering by applying cycles of
mutation, amplification and selection to genes of interest (GOIs). However, classical directed …

Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …

Cytosine base editors (CBEs) enable programmable genomic C· G-to-T· A transition
mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring …

CRISPR–Cas base-editor technology enables targeted nucleotide alterations, and is being
increasingly used for research and potential therapeutic applications,. The most widely used …

The spontaneous deamination of cytosine is a major source of transitions from C• G to T• A
base pairs, which account for half of known pathogenic point mutations in humans. The …

Recently developed DNA base editing methods enable the direct generation of desired
point mutations in genomic DNA without generating any double-strand breaks,–, but the …

Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-
G alterations,,–. However, we recently demonstrated that they can also induce transcriptome …

Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …

CRISPR-Cas–guided base editors convert A• T to G• C, or C• G to T• A, in cellular DNA for
precision genome editing. To understand the molecular basis for DNA adenosine …