CRISPR technology: A decade of genome editing is only the beginning
JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
An efficient CRISPR–Cas12a promoter editing system for crop improvement
J Zhou, G Liu, Y Zhao, R Zhang, X Tang, L Li, X Jia… - Nature Plants, 2023 - nature.com
Promoter editing represents an innovative approach to introduce quantitative trait variation
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …
PAM-less plant genome editing using a CRISPR–SpRY toolbox
Q Ren, S Sretenovic, S Liu, X Tang, L Huang, Y He… - Nature plants, 2021 - nature.com
The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …
A multipurpose toolkit to enable advanced genome engineering in plants
T Čermák, SJ Curtin, J Gil-Humanes, R Čegan… - The Plant …, 2017 - academic.oup.com
We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …
and dicot genomes using a variety of genome engineering approaches. Our reagents …
A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants
Clustered regularly interspaced short palindromic repeats (CRISPR)–Cpf1 has emerged as
an effective genome editing tool in animals. Here we compare the activity of Cpf1 from …
an effective genome editing tool in animals. Here we compare the activity of Cpf1 from …
Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
Gene editing in plants: progress and challenges
Y Mao, JR Botella, Y Liu, JK Zhu - National Science Review, 2019 - academic.oup.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
Towards CRISPR/Cas crops–bringing together genomics and genome editing
With the rapid increase in the global population and the impact of climate change on
agriculture, there is a need for crops with higher yields and greater tolerance to abiotic …
agriculture, there is a need for crops with higher yields and greater tolerance to abiotic …