The construction and assembly of artificial allosteric protein switches into information and
energy processing networks connected to both biological and non-biological systems is a …

Focusing on an important biomedical implication of allostery–design of allosteric drugs, we
describe characteristics of allosteric sites, effectors, and their modes of actions …

There were several significant milestones in the last century, from the discovery of the Bohr
effect [1] to understanding that every dynamic protein can be inherently allosteric [2, 3]. It …

Eukaryotic cell-free protein expression systems enable rapid production of recombinant
multidomain proteins in their functional form. A cell-free system based on the rapidly growing …

Post-translational modifications (PTMs) such as phosphorylation and dephosphorylation can
rapidly alter protein surface chemistry and structural conformation, which can switch protein …

Protein biosensors have emerged as valuable tools in biotechnology, offering efficient
detection and differentiation of various chemical structures. The insertion of receptor …

This thesis reports a new platform for engineering and optimisation of protein switches. The
developed all-in-vitro protein expression and screening platform allows multiplexed …