Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods

AA Pérez, G Vazquez-Meves, ME Hunter - The CRISPR Journal, 2024 - liebertpub.com
Wildlife diseases are a considerable threat to human health, conservation, and the
economy. Surveillance is a critical component to mitigate the impact of animal diseases in …

A CRISPR-Cas12a-based assay for one-step preamplification-free detection of viral DNA

Y Chen, X Ma, L Pan, S Yang, X Chen, F Wang… - Sensors and Actuators B …, 2024 - Elsevier
How to realize fM-level detection of viral DNA without target amplification is a challenge.
Traditionally, preamplification of low-abundant DNA targets is a prerequisite, which not only …

A novel CRISPR/Cas13a biosensing platform comprising dual hairpin probe and traditional lateral flow assays

H Sun, S Bu, C Wang, J Wang, Y Gao, M Xu… - Sensors and Actuators B …, 2025 - Elsevier
Abstract Recently, CRISPR/Cas13a-based biosensors have been combined with non-
traditional lateral flow assays (N-TLFAs) successfully used to clinical detection of target RNA …