Single-cell approaches in human microbiome research

V Lloréns-Rico, JA Simcock, GRB Huys, J Raes - Cell, 2022 - cell.com
Microbial culturing and meta-omic profiling technologies have significantly advanced our
understanding of the taxonomic and functional variation of the human microbiome and its …

[HTML][HTML] Culturomics of the plant prokaryotic microbiome and the dawn of plant-based culture media–a review

MS Sarhan, MA Hamza, HH Youssef, S Patz… - Journal of Advanced …, 2019 - Elsevier
Improving cultivability of a wider range of bacterial and archaeal community members, living
natively in natural environments and within plants, is a prerequisite to better understanding …

Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons

G Kapinusova, MA Lopez Marin, O Uhlik - Frontiers in Microbiology, 2023 - frontiersin.org
In terms of the number and diversity of living units, the prokaryotic empire is the most
represented form of life on Earth, and yet it is still to a significant degree shrouded in …

Anaerobic single‐cell dispensing facilitates the cultivation of human gut bacteria

A Afrizal, TCA Hitch, A Viehof, N Treichel… - Environmental …, 2022 - Wiley Online Library
Cultivation via classical agar plate (CAP) approaches is widely used to study microbial
communities, but they are time‐consuming. An alternative approach is the application of …

One cell at a time: droplet-based microbial cultivation, screening and sequencing

B Hu, P Xu, L Ma, D Chen, J Wang, X Dai… - Marine life science & …, 2021 - Springer
Microbes thrive and, in turn, influence the earth's environment, but most are poorly
understood because of our limited capacity to reveal their natural diversity and function …

Dynamics of the intestinal bacterial community in black soldier fly larval guts and its influence on insect growth and development

XY Li, C Mei, XY Luo, D Wulamu, S Zhan… - Insect …, 2023 - Wiley Online Library
Black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is a prominent insect for
the bioconversion of various organic wastes. As a saprotrophic insect, the BSF inhabits …

Emerging microfluidic technologies for microbiome research

Y Yu, H Wen, S Li, H Cao, X Li, Z Ma, X She… - Frontiers in …, 2022 - frontiersin.org
The importance of the microbiome is increasingly prominent. For example, the human
microbiome has been proven to be strongly associated with health conditions, while the …

High-throughput single-cell cultivation reveals the underexplored rare biosphere in deep-sea sediments along the Southwest Indian Ridge

B Hu, B Xu, J Yun, J Wang, B Xie, C Li, Y Yu, Y Lan… - Lab on a Chip, 2020 - pubs.rsc.org
Microorganisms in the deep sea play vital roles in marine ecosystems. However, despite
great advances brought by high throughput sequencing and metagenomics, only a small …

Recent advances in droplet microfluidics for microbiology

Z He, H Wu, X Yan, W Liu - Chinese Chemical Letters, 2022 - Elsevier
Advances in microbiology rely on innovations in technology. Droplet microfluidics, as a
versatile and powerful technique that allows high-throughput generation and manipulation …

Bacteria cultivated from sponges and bacteria not yet cultivated from sponges—A review

TTH Dat, G Steinert, NTK Cuc, H Smidt… - Frontiers in …, 2021 - frontiersin.org
The application of high-throughput microbial community profiling as well as “omics”
approaches unveiled high diversity and host-specificity of bacteria associated with marine …