Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis

H Perron, JA Garson, F Bedin… - Proceedings of the …, 1997 - National Acad Sciences
The partial molecular characterization of multiple sclerosis (MS)-associated retrovirus
(MSRV), a novel retrovirus previously called LM7, is reported. MSRV has been isolated …

Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR

WA Al-Soud, LJ Jönsson… - Journal of clinical …, 2000 - Am Soc Microbiol
ABSTRACT A major inhibitor of diagnostic PCR in human plasma was identified and the
mechanism of inhibition was characterized. Human blood was divided by centrifugation into …

iSCAN-V2: A one-pot RT-RPA–CRISPR/Cas12b assay for point-of-care SARS-CoV-2 detection

R Aman, T Marsic, G Sivakrishna Rao… - … in Bioengineering and …, 2022 - frontiersin.org
Rapid, specific, and sensitive detection platforms are prerequisites for early pathogen
detection to efficiently contain and control the spread of contagious diseases. Robust and …

Viral diseases of mulberry silkworm, Bombyx mori L.-A review

A Sharma, P Sharma, J Thakur, S Murali… - … of Pharmacognosy and …, 2020 - phytojournal.com
The mulberry silkworm, Bombyx mori (Linnaeus)(Lepidoptera: Bombycidae) is an
economically important insect domesticated for silk production. One of the main reasons for …

Inhibitory effects of green tea polyphenol (–)‐epigallocatechin gallate on the expression of matrix metalloproteinase‐9 and on the formation of osteoclasts

JH Yun, EK Pang, CS Kim, YJ Yoo… - Journal of …, 2004 - Wiley Online Library
Background: Alveolar bone resorption is a characteristic feature of periodontal diseases and
involves the removal of both the mineral and organic constituents of the bone matrix, which …

PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency

O Suslov, DA Steindler - Nucleic acids research, 2005 - academic.oup.com
This study addresses the problem of PCR inhibition by reverse transcriptase. It has been
shown that the inhibition occurs mostly when a small amount of RNA is taken for RT …

Molecular epizootiology of avian infectious bronchitis in Russia

YA Bochkov, GV Batchenko, LO Shcherbakova… - Avian …, 2006 - Taylor & Francis
Molecular characterization of infectious bronchitis viruses (IBVs) isolated between 1998 and
2002 from chickens in Russia was performed. More than 250 field samples were tested by …

Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR

DP Chandler, CA Wagnon… - Applied and …, 1998 - Am Soc Microbiol
Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while
developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and …

Alteration of enzymes and their application to nucleic acid amplification

K Yasukawa, I Yanagihara… - … Journal of Molecular …, 2020 - spandidos-publications.com
Since the discovery of polymerase chain reaction (PCR) in 1985, several methods have
been developed to achieve nucleic acid amplification, and are currently used in various …

Reverse transcriptase

GF Gerard - enzymes of nucleic acid synthesis and modification, 2018 - taylorfrancis.com
Retrovirus reverse transcriptase has been the subject of intense investigation since its
discovery in 1970. Retroviruses and their resident reverse transcriptases have been …