CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

In the last years, tremendous progress has been made in the development of CRISPR/Cas-
mediated genome editing tools. A number of natural CRISPR/Cas nuclease variants have …

Highlights●Adapting new nucleases and improving established ones expand the
CRISPR/Cas toolbox.●Optimized base editors enlarge editing windows and enable CG …

Increasing crop production to meet the demands of a growing population depends largely
on crop improvement through new plant-breeding techniques (NPBT) such as genome …

Targeted genome editing technologies are becoming the most important and widely used
genetic tools in studies of phytopathology. The “clustered regularly interspaced short …

The class II clustered regularly interspaced short palindromic repeats (CRISPR)–Cas
systems, characterized by a single effector protein, can be further subdivided into types II, V …

Gene editing techniques, which help in modification of any DNA sequence at ease, have
revolutionized the world of Genetic engineering. Although there are other gene-editing …

Vegetable crops are known as protective foods due to their potential role in a balanced
human diet, especially for vegetarians as they are a rich source of vitamins and minerals …

Genome editing is an important strategy to maintain global food security and achieve
sustainable agricultural development. Among all genome editing tools, CRISPR-Cas is …