The ability to design efficient enzymes from scratch would have a profound effect on
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …

Metalloenzymes catalyze a variety of reactions using a limited number of natural amino
acids and metallocofactors. Therefore, the environment beyond the primary coordination …

The incorporation of a synthetic, catalytically competent metallocofactor into a protein
scaffold to generate an artificial metalloenzyme (ArM) has been explored since the late …

Proteins are molecular machines whose function depends on their ability to achieve
complex folds with precisely defined structural and dynamic properties. The rational design …

One of the hallmark advances in our understanding of metalloprotein function is showcased
in our ability to design new, non-native, catalytically active protein scaffolds. This review …

Protein design is a valuable tool for understanding the fundamental factors that dictate
protein structure and function. The field of protein design has seen significant progress over …

Selective metal coordination is central to the functions of metalloproteins:, each
metalloprotein must pair with its cognate metallocofactor to fulfil its biological role. However …

Enzymes are extremely complex catalytic structures with immense biological and
technological importance. Nevertheless, their widespread environmental implementation …

We present here a broad overview of the field of metabolic engineering, describing in the
first section the key fundamental principles that define and distinguish it, as well as the …

Metal ions are an important part of many natural proteins, providing structural, catalytic and
electron transfer functions. Reproducing these functions in a designed protein is the ultimate …