Speed scaling in multiphoton fluorescence microscopy

J Wu, N Ji, KK Tsia - Nature Photonics, 2021 - nature.com
After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …

In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope

J Boguslawski, G Palczewska… - The Journal of …, 2022 - Am Soc Clin Investig
Background Noninvasive assessment of metabolic processes that sustain regeneration of
human retinal visual pigments (visual cycle) is essential to improve ophthalmic diagnostics …

Mode-Locking Dynamics in an All-PM Figure-Nine Tm-Doped Fiber Laser

Z Łaszczych, M Krakowski, G Soboń - Applied Sciences, 2022 - mdpi.com
We report a study on pulse dynamics in figure-nine Tm-doped all-polarization maintaining
fiber laser. We analyzed laser operation from self-starting with multi-pulse dynamic to single …

Increasing brightness in multiphoton microscopy with a low-repetition-rate, wavelength-tunable femtosecond fiber laser

J Bogusławski, A Kwaśny, D Stachowiak… - Optics …, 2023 - opg.optica.org
Many experiments in biological and medical sciences currently use multiphoton microscopy
as a core imaging technique. To date, solid-state lasers are most commonly used as …

Femtosecond Er-doped fiber laser source tunable from 872 to 1075 nm for two-photon vision studies in humans

D Stachowiak, M Marzejon, J Bogusławski… - Biomedical Optics …, 2022 - opg.optica.org
We report the development of a widely-tunable femtosecond fiber laser system and its
application for two-photon vision studies. The source is based on an Er-doped fiber laser …

Nonlinear chirped pulse amplification for a 100-W-class GHz femtosecond all-fiber laser system at 1.5 m

Y Fan, H Xiu, W Lin, X Chen, X Hu, W Wang… - High Power Laser …, 2023 - cambridge.org
In this work, we present a high-power, high-repetition-rate, all-fiber femtosecond laser
system operating at 1.5 m, to the best of our knowledge. By utilizing the soliton-effect-based …

[HTML][HTML] In vivo imaging of the human retina using a two-photon excited fluorescence ophthalmoscope

J Bogusławski, S Tomczewski, M Dąbrowski, K Komar… - STAR protocols, 2023 - Elsevier
Noninvasive imaging of endogenous retinal fluorophores, including vitamin A derivatives, is
vital to developing new treatments for retinal diseases. Here, we present a protocol for …

Synthesis of hexagonal structured GaS nanosheets for robust femtosecond pulse generation

K Guo, Q Yu, F Liu, H Deng, T Yi, B Ren, W Su, S Zhu… - Nanomaterials, 2022 - mdpi.com
Gallium sulfide (GaS), with a hexagonal structure, has received extensive attention due to its
graphene-like structure and derived optical properties. Here, high-quality GaS was obtained …

Pulse train gating to improve signal generation for in vivo two-photon fluorescence microscopy

SA Engelmann, A Tomar, AL Woods… - …, 2023 - spiedigitallibrary.org
Significance Two-photon microscopy is used routinely for in vivo imaging of neural and
vascular structures and functions in rodents with a high resolution. Image quality, however …

Efficient multiphoton microscopy with picosecond laser pulses

K Kunio, J Bogusławski, G Soboń - Optics Letters, 2024 - opg.optica.org
Multiphoton microscopes employ femtosecond lasers as light sources because the high
peak power of the ultrashort pulse allows for multiphoton excitation of fluorescence in the …