Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and 1H Detection
Magic angle spinning (MAS) NMR studies of amyloid and membrane proteins and large
macromolecular complexes are an important new approach to structural biology. However …
macromolecular complexes are an important new approach to structural biology. However …
New applications of solid-state NMR in structural biology
PCA van der Wel - Emerging topics in life sciences, 2018 - portlandpress.com
Various recent developments in solid-state nuclear magnetic resonance (ssNMR)
spectroscopy have enabled an array of new insights regarding the structure, dynamics, and …
spectroscopy have enabled an array of new insights regarding the structure, dynamics, and …
Dangerous liaisons between detergents and membrane proteins. The case of mitochondrial uncoupling protein 2
The extraction of membrane proteins from their native environment by detergents is central
to their biophysical characterization. Recent studies have emphasized that detergents may …
to their biophysical characterization. Recent studies have emphasized that detergents may …
Solid-state NMR structure determination from diagonal-compensated, sparsely nonuniform-sampled 4D proton–proton restraints
We report acquisition of diagonal-compensated protein structural restraints from four-
dimensional solid-state NMR spectra on extensively deuterated and 1H back-exchanged …
dimensional solid-state NMR spectra on extensively deuterated and 1H back-exchanged …
Amphipols outperform dodecylmaltoside micelles in stabilizing membrane protein structure in the gas phase
AN Calabrese, TG Watkinson, PJF Henderson… - Analytical …, 2015 - ACS Publications
Noncovalent mass spectrometry (MS) is emerging as an invaluable technique to probe the
structure, interactions, and dynamics of membrane proteins (MPs). However, maintaining …
structure, interactions, and dynamics of membrane proteins (MPs). However, maintaining …
Dynamic nuclear polarization as an enabling technology for solid state nuclear magnetic resonance spectroscopy
AN Smith, JR Long - Analytical chemistry, 2016 - ACS Publications
Magic angle spinning (MAS) solid state nuclear magnetic resonance spectroscopy (ssNMR)
can yield unique and insightful information for complex systems; most notably structural and …
can yield unique and insightful information for complex systems; most notably structural and …
Structure determination of membrane proteins in their native phospholipid bilayer environment by rotationally aligned solid-state NMR spectroscopy
SJ Opella - Accounts of chemical research, 2013 - ACS Publications
One of the most important topics in experimental structural biology is determining the
structures of membrane proteins. These structures represent one-third of all of the …
structures of membrane proteins. These structures represent one-third of all of the …
Structure of CrgA, a cell division structural and regulatory protein from Mycobacterium tuberculosis, in lipid bilayers
The 93-residue transmembrane protein CrgA in Mycobacterium tuberculosis is a central
component of the divisome, a large macromolecular machine responsible for cell division …
component of the divisome, a large macromolecular machine responsible for cell division …
Gating mechanism of aquaporin Z in synthetic bilayers and native membranes revealed by solid-state NMR spectroscopy
Aquaporin Z (AqpZ) is an integral membrane protein that facilitates transport of water across
Escherichia coli cells with a high rate. Previously, R189, a highly conserved residue of the …
Escherichia coli cells with a high rate. Previously, R189, a highly conserved residue of the …
NMR structures of membrane proteins in phospholipid bilayers
Membrane proteins have always presented technical challenges for structural studies
because of their requirement for a lipid environment. Multiple approaches exist including X …
because of their requirement for a lipid environment. Multiple approaches exist including X …