Real-time polymerase chain reaction: Current techniques, applications, and role in COVID-19 diagnosis

IM Artika, YP Dewi, IM Nainggolan, JE Siregar… - Genes, 2022 - mdpi.com
Successful detection of the first SARS-CoV-2 cases using the real-time polymerase chain
reaction (real-time PCR) method reflects the power and usefulness of this technique. Real …

Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum

CH Choi, E Kim, SM Yang, DS Kim, SM Suh, GY Lee… - Foods, 2022 - mdpi.com
Droplet digital polymerase chain reaction (ddPCR) is one of the newest and most promising
tools providing absolute quantification of target DNA molecules. Despite its emerging …

[HTML][HTML] ddPCR strategy to detect a gene-edited plant carrying a single variation point: Technical feasibility and interpretation issues

MA Fraiture, E Guiderdoni, AC Meunier, N Papazova… - Food Control, 2022 - Elsevier
Gene-edited organisms and derived food and feed products commercialized on the
European market falls within the scope of the Directive, 2001/18/EC. Therefore, the …

Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products

J D'aes, MA Fraiture, B Bogaerts… - Life, 2022 - mdpi.com
Genetically modified microorganisms (GMM) are frequently employed for manufacturing
microbial fermentation products such as food enzymes or vitamins. Although the …

[HTML][HTML] Pilot market surveillance of GMM contaminations in alpha-amylase food enzyme products: A detection strategy strengthened by a newly developed qPCR …

MA Fraiture, A Gobbo, C Guillitte, U Marchesi… - Food Chemistry …, 2024 - Elsevier
Using high-throughput metagenomics on commercial microbial fermentation products, DNA
from a new unauthorized genetically modified microorganism (GMM), namely the GM B …

[HTML][HTML] Recombinant DNA in fermentation products is of no regulatory relevance

A Lensch, E Duwenig, HG Dederer, SO Kärenlampi… - Food Control, 2022 - Elsevier
A large variety of fermentation products are used in food and feed production, but also in
other industries, and many of these products are produced with genetically modified …

[HTML][HTML] Metagenomics-based tracing of genetically modified microorganism contaminations in commercial fermentation products

J D'aes, MA Fraiture, B Bogaerts, Y Van Laere… - Food Chemistry …, 2024 - Elsevier
Genetically modified microorganisms (GMM) are frequently employed for the production of
microbial fermentation products such as food enzymes. Although presence of the GMM or its …

Development and collaborative validation of an event-specific quantitative real-time PCR method for detection of genetically modified CC-2 maize

L Long, N Zhao, C Li, Y He, L Dong, W Yan… - Frontiers in Plant …, 2024 - frontiersin.org
As one of the developed genetically modified (GM) maize varieties in China, CC-2 has
demonstrated promising commercial prospects during demonstration planting. The …

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

MA Fraiture, A Gobbo, N Papazova, NHC Roosens - Fermentation, 2023 - mdpi.com
Recently, a genetically modified microorganism (GMM) detection strategy using real-time
PCR technology was developed to control fermentation products commercialized in the food …

Development of a Taxon-Specific Real-Time PCR Method Targeting the Bacillus subtilis Group to Strengthen the Control of Genetically Modified Bacteria in …

MA Fraiture, A Gobbo, N Papazova, NHC Roosens - Fermentation, 2022 - mdpi.com
Most of the bacteria that are used to produce fermentation products, such as enzymes,
additives and flavorings, belong to the Bacillus subtilis group. Recently, unexpected …