Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Second and outer coordination sphere effects in nitrogenase, hydrogenase, formate dehydrogenase, and CO dehydrogenase
Gases like H2, N2, CO2, and CO are increasingly recognized as critical feedstock in “green”
energy conversion and as sources of nitrogen and carbon for the agricultural and chemical …
energy conversion and as sources of nitrogen and carbon for the agricultural and chemical …
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …
screening. The success of such campaigns is crucially dependent on the number of …
Advances in ultrahigh-throughput screening for directed enzyme evolution
U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …
technology platform for high-throughput protein production and genetic part characterization …
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
T Jaroentomeechai, JC Stark, A Natarajan… - Nature …, 2018 - nature.com
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
Cell-free protein synthesis: applications come of age
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the
growing demand for simple and efficient protein production. While used for decades as a …
growing demand for simple and efficient protein production. While used for decades as a …
Droplet microfluidics for microbiology: techniques, applications and challenges
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …
Bottom-up construction of complex biomolecular systems with cell-free synthetic biology
N Laohakunakorn, L Grasemann… - … in bioengineering and …, 2020 - frontiersin.org
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
High throughput screening and selection methods for directed enzyme evolution
Successful evolutionary enzyme engineering requires a high throughput screening or
selection method, which considerably increases the chance of obtaining desired properties …
selection method, which considerably increases the chance of obtaining desired properties …