Microbiome and human health: current understanding, engineering, and enabling technologies

N Aggarwal, S Kitano, GRY Puah, S Kittelmann… - Chemical …, 2022 - ACS Publications
The human microbiome is composed of a collection of dynamic microbial communities that
inhabit various anatomical locations in the body. Accordingly, the coevolution of the …

Opportunities and challenges of using metagenomic data to bring uncultured microbes into cultivation

S Liu, CD Moon, N Zheng, S Huws, S Zhao, J Wang - Microbiome, 2022 - Springer
Although there is now an extensive understanding of the diversity of microbial life on earth
through culture-independent metagenomic DNA sequence analyses, the isolation and …

Recent advanced technologies for the characterization of xenobiotic-degrading microorganisms and microbial communities

S Mishra, Z Lin, S Pang, W Zhang, P Bhatt… - … in Bioengineering and …, 2021 - frontiersin.org
Global environmental contamination with a complex mixture of xenobiotics has become a
major environmental issue worldwide. Many xenobiotic compounds severely impact the …

Advances and challenges in metatranscriptomic analysis

M Shakya, CC Lo, PSG Chain - Frontiers in genetics, 2019 - frontiersin.org
Sequencing-based analyses of microbiomes have traditionally focused on addressing the
question of community membership and profiling taxonomic abundance through amplicon …

Perspectives and benefits of high-throughput long-read sequencing in microbial ecology

L Tedersoo, M Albertsen, S Anslan… - Applied and …, 2021 - Am Soc Microbiol
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

The soil microbiome—from metagenomics to metaphenomics

JK Jansson, KS Hofmockel - Current opinion in microbiology, 2018 - Elsevier
Soil microorganisms carry out important processes, including support of plant growth and
cycling of carbon and other nutrients. However, the majority of soil microbes have not yet …

Accurate and complete genomes from metagenomes

LX Chen, K Anantharaman, A Shaiber… - Genome …, 2020 - genome.cshlp.org
Genomes are an integral component of the biological information about an organism; thus,
the more complete the genome, the more informative it is. Historically, bacterial and …

The multi-omics promise in context: from sequence to microbial isolate

J Gutleben, M Chaib De Mares… - Critical reviews in …, 2018 - Taylor & Francis
The numbers and diversity of microbes in ecosystems within and around us is unmatched,
yet most of these microorganisms remain recalcitrant to in vitro cultivation. Various high …

Moisture modulates soil reservoirs of active DNA and RNA viruses

R Wu, MR Davison, Y Gao, CD Nicora… - Communications …, 2021 - nature.com
Soil is known to harbor viruses, but the majority are uncharacterized and their responses to
environmental changes are unknown. Here, we used a multi-omics approach …

Metagenomic assembly through the lens of validation: recent advances in assessing and improving the quality of genomes assembled from metagenomes

ND Olson, TJ Treangen, CM Hill… - Briefings in …, 2019 - academic.oup.com
Metagenomic samples are snapshots of complex ecosystems at work. They comprise
hundreds of known and unknown species, contain multiple strain variants and vary greatly …