The ongoing development of HPLC has been focused on increasing the speed and
efficiency of separations over the past decade. The advances in separation speed have …

Reversed phase liquid chromatography using superheated water as the mobile phase, at
temperatures between 100 and 250° C, offers a number of advantages for the analyst. It is …

Intact polar lipid distributions have become a valuable tool for the study of microbial
ecosystems. In order to expand the detection and interpretation of the presence of these …

The properties of silica-based, chemically bonded, packed column stationary phases used
in supercritical fluid chromatography are described with a focus on column design and …

Abstract An un‐derivatized 1.7 μm ethylene bridged hybrid (BEH) particle was evaluated for
its utility in retaining polar species in hydrophilic interaction chromatography (HILIC), and …

Free silanols on the surface of silica are the “villains”, which are responsible for detrimental
interactions of those compounds and the stationary phase (ie, bad peak shape, low …

Oligonucleotides represent one of the most significant pharmaceutical breakthroughs in
recent years, showing great promise as diagnostic and therapeutic agents for malignant …

Simple, robust and versatile LC-MS based methods add to the rapid assessment of the
lipidome of biological cells. Here we present a versatile RP-UHPLC-MS method using 1 …

Ionic analytes, such as peptides, can be challenging to separate by reverse-phase
chromatography with optimal efficiency. They tend, for instance, to exhibit poor peak shapes …

The influence of the mobile phase on retention is studied in this paper for a group of over 70
compounds with a broad range of multiple functional groups. We varied the pH of the mobile …