Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry

JD Dunn, GE Reid, ML Bruening - Mass spectrometry reviews, 2010 - Wiley Online Library
Mass spectrometry is the tool of choice to investigate protein phosphorylation, which plays a
vital role in cell regulation and diseases such as cancer. However, low abundances of …

Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications

J Huang, F Wang, M Ye, H Zou - Journal of Chromatography A, 2014 - Elsevier
Comprehensive analysis of the post-translational modifications (PTMs) on proteins at
proteome level is crucial to elucidate the regulatory mechanisms of various biological …

[HTML][HTML] Deciphering plant-microbe crosstalk through proteomics studies

A Jain, HB Singh, S Das - Microbiological Research, 2021 - Elsevier
Proteomic approaches are being used to elucidate a better discretion of interactions
occurring between host, pathogen, and/or beneficial microorganisms at the molecular level …

Comparison of in‐gel protein separation techniques commonly used for fractionation in mass spectrometry‐based proteomic profiling

M Jafari, V Primo, GB Smejkal, EV Moskovets… - …, 2012 - Wiley Online Library
Fractionation of complex samples at the cellular, subcellular, protein, or peptide level is an
indispensable strategy to improve the sensitivity in mass spectrometry‐based proteomic …

[HTML][HTML] Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin …

B Rahmutulla, K Matsushita, M Satoh, M Seimiya… - Oncotarget, 2014 - ncbi.nlm.nih.gov
The far-upstream element-binding protein-interacting repressor (FIR) is a c-myc
transcriptional suppressor. FIR is alternatively spliced to lack the transcriptional repression …

[HTML][HTML] A novel role for survivin in erythroblast enucleation

G Keerthivasan, H Liu, JM Gump, SF Dowdy… - …, 2012 - ncbi.nlm.nih.gov
Background Nucleus free red blood cells are unique to mammals. During their terminal
stage of differentiation, mammalian erythroblasts exit the cell cycle and enucleate. We …

SAP155-Mediated Splicing of FUSE-Binding Protein-Interacting Repressor Serves as a Molecular Switch for c-myc Gene Expression

K Matsushita, T Kajiwara, M Tamura, M Satoh… - Molecular Cancer …, 2012 - AACR
Abstract The Far UpStream Element (FUSE)-binding protein-interacting repressor (FIR), a c-
myc transcriptional suppressor, is alternatively spliced removing the transcriptional …

Yeast proteomics and protein microarrays

R Chen, M Snyder - Journal of proteomics, 2010 - Elsevier
Our understanding of biological processes as well as human diseases has improved greatly
thanks to studies on model organisms such as yeast. The power of scientific approaches …

Using multiplex-staining to study changes in the maize leaf phosphoproteome in response to mechanical wounding

E Lewandowska-Gnatowska, ML Johnston, W Antoine… - Phytochemistry, 2011 - Elsevier
Mechanical wounding of 2-week-old maize (Zea mays L.) leaves, one of the first steps in
both pathogen infection and herbivore attack, stimulates metabolism and activates signal …

[HTML][HTML] Protein mobility shifts contribute to gel electrophoresis liquid chromatography analysis

NJ Carruthers, GC Parker, T Gratsch… - Journal of …, 2015 - ncbi.nlm.nih.gov
Profiling of cellular and subcellular proteomes by liquid chromatography with tandem mass
spectrometry (MS) after fractionation by SDS-PAGE is referred to as GeLC (gel …