A step towards validation of high-throughput sequencing for the identification of plant pathogenic oomycetes

AS Espindola, K Cardwell, FN Martin… - …, 2022 - Am Phytopath Society
The advancement in high-throughput sequencing (HTS) technology allows the detection of
pathogens without the need for isolation or template amplification. Plant regulatory agencies …

TASPERT: Target-specific reverse transcript pools to improve HTS plant virus diagnostics

AS Espindola, D Sempertegui-Bayas, DF Bravo-Padilla… - Viruses, 2021 - mdpi.com
High-throughput sequencing (HTS) is becoming the new norm of diagnostics in plant
quarantine settings. HTS can be used to detect, in theory, all pathogens present in any given …

Microbe Finder (MiFi®): Implementation of an Interactive Pathogen Detection Tool in Metagenomic Sequence Data

AS Espindola, KF Cardwell - Plants, 2021 - mdpi.com
Agricultural high throughput diagnostics need to be fast, accurate and have multiplexing
capacity. Metagenomic sequencing is being widely evaluated for plant and animal …

Detection of Phytophthora, Pythium, Globisporangium, Hyaloperonospora, and Plasmopara Species in High-Throughput Sequencing Data by In Silico and In Vitro …

F Proaño-Cuenca, AS Espindola… - PhytoFrontiers …, 2023 - Am Phytopath Society
Species identification of plant pathogenic oomycetes based on morphology is challenging
because of the limited number of variable characters available. Hence, pure culture isolation …

Development and Validation of E-Probes with the MiFi System for Detection of Ralstonia solanacearum Species Complex in Blueberries

AM Bocsanczy, AS Espindola, K Cardwell… - PhytoFrontiers …, 2023 - Am Phytopath Society
The blueberry industry, a fast-growing industry in Florida, is threatened by bacterial wilt,
caused by the Ralstonia solanacearum species complex (RSSC). In 2016, distinct RSSC …