Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …

Recent developments in computational chemistry and biology have come together in the
“inside‐out” approach to enzyme engineering. Proteins have been designed to catalyze …

Asymmetric catalysis plays a key role in modern synthetic organic chemistry, with synthetic
catalysts and enzymes being the two available options. During the latter part of the last …

Biocatalysis has emerged as a powerful alternative to traditional chemistry, especially for
asymmetric synthesis. One key requirement during process development is the discovery of …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

Our previous contribution to increasing the efficiency of directed evolution is iterative
saturation mutagenesis (ISM) as a systematic means of generating focused libraries for the …

This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Directed evolution can generate a remarkable range of new enzyme properties. Alternate
substrate specificities and reaction selectivities are readily accessible in enzymes from …

Engineering enzymes to degrade anthropogenic compounds efficiently is challenging. We
obtained Rhodococcus rhodochrous haloalkane dehalogenase mutants with up to 32-fold …