Cryopreservation techniques exist for some 100 plant species. Cryopreservation for the long-
term conservation of in vitro germplasm results in the exposure of tissues to physical …

Rapid increases in human populations provide a great challenge to ensure that adequate
quantities of food are available. Sustainable development of agricultural production by …

The aim of the present study was to apply cryopreservation for the embryogenic cultures of
open-pollinated Abies cephalonica, as well as to test the genetic fidelity of the cryopreserved …

Scots pine (Pinus sylvestris L.) is an economically very important and scientifically well
investigated tree species of the northern hemisphere occupying large areas with different …

The aim of this study is to investigate the feasibility of using well defined, serum‐free
freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2.5%(v/v) …

Several factors affecting somatic embryogenesis (SE) in Pinus sylvestris from self-and cross-
pollinated seed families were studied with the aim of producing large quantities of clonal …

An efficient short term storage protocol was developed for Ansellia africana, a vulnerable
medicinal orchid of Africa using encapsulated protocorm-like bodies (PLBs) induced from …

Simple Summary Optimization of regrowth conditions is critical for the successful
cryopreservation of in vitro plant germplasm. This review discusses the five major strategies …

Questions often arise concerning the genetic stability of plant materials stored in liquid
nitrogen for long time periods. This study examined the genetic stability of cryopreserved …

A vitrification based cryopreservation technique for storage of in vitro shoot tips of papaya
has been tested to ensure applicability across a range of genotypes and to assess the …