The promise and challenge of high-throughput sequencing of the antibody repertoire
G Georgiou, GC Ippolito, J Beausang, CE Busse… - Nature …, 2014 - nature.com
Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid
organs using high-throughput DNA sequencing technologies have been advancing at an …
organs using high-throughput DNA sequencing technologies have been advancing at an …
Single cell transcriptomics: methods and applications
Traditionally, gene expression measurements were performed on “bulk” samples containing
populations of thousands of cells. Recent advances in genomic technology have made it …
populations of thousands of cells. Recent advances in genomic technology have made it …
Combinatorial labeling of single cells for gene expression cytometry
INTRODUCTION The measurement of specific proteins and transcripts in individual cells is
critical for understanding the role of cellular diversity in development, health, and disease …
critical for understanding the role of cellular diversity in development, health, and disease …
Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a
versatile technology that enables multiplexed amplicon sequencing and transcriptome …
versatile technology that enables multiplexed amplicon sequencing and transcriptome …
Quantitative assessment of the robustness of next-generation sequencing of antibody variable gene repertoires from immunized mice
Background Next-generation sequencing (NGS) of antibody variable regions has emerged
as a powerful tool in systems immunology by providing quantitative molecular information on …
as a powerful tool in systems immunology by providing quantitative molecular information on …
Comprehensive evaluation and optimization of amplicon library preparation methods for high-throughput antibody sequencing
High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful
tool in the field of systems immunology. However, numerous sources of bias in HTS …
tool in the field of systems immunology. However, numerous sources of bias in HTS …
Antibody repertoire analysis of mouse immunization protocols using microfluidics and molecular genomics
MA Asensio, YW Lim, N Wayham, K Stadtmiller… - MAbs, 2019 - Taylor & Francis
Immunization of mice followed by hybridoma or B-cell screening is one of the most common
antibody discovery methods used to generate therapeutic monoclonal antibody (mAb) …
antibody discovery methods used to generate therapeutic monoclonal antibody (mAb) …
N 6-Methyladenosine Methylomic Landscape of Lung Tissues in Murine Acute Allergic Asthma
F Teng, W Tang, T Wuniqiemu, J Qin, Y Zhou… - Frontiers in …, 2021 - frontiersin.org
Allergic asthma is well known as a common respiratory disorder comprising an allergic
inflammatory nature and excessive immune characteristic. N 6-methyladenosine (m6A) …
inflammatory nature and excessive immune characteristic. N 6-methyladenosine (m6A) …
[HTML][HTML] PoliViews: A comprehensive and modular approach to the conceptual modeling of genomic data
The human genome complexity is captured by many signals, representing for instance DNA
variations, the expression of gene activity, or DNA's structural rearrangements; a rich set of …
variations, the expression of gene activity, or DNA's structural rearrangements; a rich set of …
Isotachophoresis for fractionation and recovery of cytoplasmic RNA and nucleus from single cells
K Kuriyama, H Shintaku, JG Santiago - Electrophoresis, 2015 - Wiley Online Library
There is a substantial need for simultaneous analyses of RNA and DNA from individual
single cells. Such analysis provides unique evidence of cell‐to‐cell differences and the …
single cells. Such analysis provides unique evidence of cell‐to‐cell differences and the …