The development of microscopy for super-resolution: Confocal microscopy, and image scanning microscopy

CJR Sheppard - Applied Sciences, 2021 - mdpi.com
Optical methods of super-resolution microscopy, such as confocal microscopy, structured
illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These …

Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

CJR Sheppard - … Transactions of the Royal Society A, 2021 - royalsocietypublishing.org
Structured illumination microscopy and image scanning microscopy are two microscopical
tech-niques, rapidly increasing in practical application, that can result in improvement in …

Signal strength and integrated intensity in confocal and image scanning microscopy

CJR Sheppard, M Castello, G Tortarolo… - Journal of the Optical …, 2022 - opg.optica.org
The properties of signal strength and integrated intensity in a scanned imaging system are
reviewed. These properties are especially applied to confocal imaging systems, including …

[PDF][PDF] 无衍射贝塞尔光束的传输调控研究进展(特邀)

李岩珂, 刘圣, 李鹏, 魏冰妍, 温丹丹, 赵建林 - Acta Optica Sinica, 2024 - researchgate.net
摘要贝塞尔光束以其独特的无衍射传输特性得到了广泛的关注, 在光学操控, 光学加工,
信息传输, 光学成像等领域具有巨大的应用前景. 为了丰富光束的传输特性 …

Pixel reassignment in image scanning microscopy with a doughnut beam: example of maximum likelihood restoration

CJR Sheppard, M Castello, G Tortarolo, E Slenders… - JOSA A, 2021 - opg.optica.org
In image scanning microscopy, the pinhole of a confocal microscope is replaced by a
detector array. The point spread function for each detector element can be interpreted as the …

Ultra-low-cost and high-fidelity NIR-II confocal laser scanning microscope with Bessel beam excitation and SiPM detection

X Wang, T Yan, L Wang, N Wang, X Yang… - Biomedical Optics …, 2024 - opg.optica.org
Confocal laser scanning microscopy (CLSM) is one of the most important imaging tools in
the biomedical field, and near-infrared-II (NIR-II, 900-1700nm) fluorescence imaging …

Extending the Depth of Focus of an Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride

M Han, D Smith, T Kahro, D Stonytė, A Kasikov… - Micromachines, 2024 - mdpi.com
Axial resolution is one of the most important characteristics of a microscope. In all
microscopes, a high axial resolution is desired in order to discriminate information efficiently …

Three Photon Excited Image Scanning Microscopy for in-Depth Super-Resolution Studies of Biological Samples

A Classen, IM Antolovic, S Vitha, M Pacheco… - PRX Life, 2024 - APS
Multiphoton laser scanning microscopy is a powerful tool for deep imaging of thick biological
samples. Image scanning microscopy (ISM) has demonstrated significant improvements in …

[图书][B] Multimodal and Nanoscale Optical Microscopy

P Bianchini, A Diaspro, CJR Sheppard - 2023 - books.google.com
Optical microscopy is developing into nanoscopy and multimodal microscopy the better to
decipher the functioning mechanisms in living systems, and investigating biological …

ISM-assisted tomographic STED microscopy

J Kratz, C Geisler, A Egner - Optics Express, 2022 - opg.optica.org
Stimulated emission depletion (STED) microscopy theoretically provides unlimited
resolution. However, in practice the achievable resolution in biological samples is …