High-throughput and single-cell T cell receptor sequencing technologies
JA Pai, AT Satpathy - Nature methods, 2021 - nature.com
T cells express T cell receptors (TCRs) composed of somatically recombined TCRα and
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
Single‐cell analysis using droplet microfluidics
Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …
Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes
The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies
that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS …
that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS …
High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics
A Gérard, A Woolfe, G Mottet, M Reichen… - Nature …, 2020 - nature.com
Mining the antibody repertoire of plasma cells and plasmablasts could enable the discovery
of useful antibodies for therapeutic or research purposes. We present a method for high …
of useful antibodies for therapeutic or research purposes. We present a method for high …
Overview of methodologies for T-cell receptor repertoire analysis
Background The T-cell receptor (TCR), located on the surface of T cells, is responsible for
the recognition of the antigen-major histocompatibility complex, leading to the initiation of an …
the recognition of the antigen-major histocompatibility complex, leading to the initiation of an …
[HTML][HTML] T-cell repertoire analysis and metrics of diversity and clonality
J Chiffelle, R Genolet, MAS Perez, G Coukos… - Current opinion in …, 2020 - Elsevier
Highlights•Immune fitness in health and disease depends notably on the diversity of cellular
repertoires.•Single-cell sequencing is fostering the understanding of clonal diversity of T-cell …
repertoires.•Single-cell sequencing is fostering the understanding of clonal diversity of T-cell …
Single cell T cell receptor sequencing: techniques and future challenges
M De Simone, G Rossetti, M Pagani - Frontiers in Immunology, 2018 - frontiersin.org
The peculiarity of T cell is their ability to recognize an infinite range of self and foreign
antigens. This ability is achieved during thymic development through a complex molecular …
antigens. This ability is achieved during thymic development through a complex molecular …
Suspendable hydrogel nanovials for massively parallel single-cell functional analysis and sorting
Techniques to analyze and sort single cells based on functional outputs, such as secreted
products, have the potential to transform our understanding of cellular biology as well as …
products, have the potential to transform our understanding of cellular biology as well as …
Hybrid immunity to SARS-CoV-2 arises from serological recall of IgG antibodies distinctly imprinted by infection or vaccination
We describe the molecular-level composition of polyclonal immunoglobulin G (IgG) anti-
spike antibodies from ancestral severe acute respiratory syndrome coronavirus 2 (SARS …
spike antibodies from ancestral severe acute respiratory syndrome coronavirus 2 (SARS …
Functional TCR T cell screening using single-cell droplet microfluidics
Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer
immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone …
immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone …