Live-cell imaging reveals the endolysosomal system as a complex and highly dynamic
network of interacting compartments. Distinct types of endosomes are discerned by kinetic …

The chapter about the Gram-positive bacterial cell wall gives a brief historical background
on the discovery of Gram-positive cell walls and their constituents and microscopic methods …

Cells store metabolic energy in the form of neutral lipids that are deposited within lipid
droplets (LDs). In this study, we examine the biogenesis of LDs and the transport of integral …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

Three‐dimensional (3D) structural analysis is essential to understand the relationship
between the structure and function of an object. Many analytical techniques, such as X‐ray …

The cell envelope of mycobacteria, which include the causative agents of tuberculosis and
leprosy, is crucial for their success as pathogens. Despite a continued strong emphasis on …

Since the beginning of the 1980s, cryo‐electron microscopy of a thin film of vitrified aqueous
suspension has made it possible to observe biological particles in their native state, in the …

High-pressure freezing of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1 in the
presence of cryoprotectants provided consistent vitrification of cells so that frozen-hydrated …

Background Transmission electron tomography is an increasingly common three-
dimensional electron microscopy approach that can provide new insights into the structure …

To fully understand biological processes from the metabolism of a bacterium to the operation
of a human brain, it is necessary to know the three-dimensional (3D) spatial arrangement …