Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research

J Gehl - Acta Physiologica Scandinavica, 2003 - Wiley Online Library
Electroporation designates the use of short high‐voltage pulses to overcome the barrier of
the cell membrane. By applying an external electric field, which just surpasses the …

Gene electrotransfer: a mechanistic perspective

C Rosazza, S Haberl Meglic, A Zumbusch… - Current gene …, 2016 - ingentaconnect.com
Gene electrotransfer is a powerful method of DNA delivery offering several medical
applications, among the most promising of which are DNA vaccination and gene therapy for …

An improved yeast transformation method for the generation of very large human antibody libraries

L Benatuil, JM Perez, J Belk… - … Engineering, Design & …, 2010 - academic.oup.com
Antibody library selection by yeast display technology is an efficient and highly sensitive
method to identify binders to target antigens. This powerful selection tool, however, is often …

[HTML][HTML] Modeling electroporation in a single cell

W Krassowska, PD Filev - Biophysical journal, 2007 - cell.com
Electroporation uses electric pulses to promote delivery of DNA and drugs into cells. This
study presents a model of electroporation in a spherical cell exposed to an electric field. The …

The molecular basis of electroporation

DP Tieleman - BMC biochemistry, 2004 - Springer
Background Electroporation is a common method to introduce foreign molecules into cells,
but its molecular basis is poorly understood. Here I investigate the mechanism of pore …

A brief overview of electroporation pulse strength–duration space: A region where additional intracellular effects are expected

JC Weaver, KC Smith, AT Esser, RS Son… - …, 2012 - Elsevier
Electroporation (EP) of outer cell membranes is widely used in research, biotechnology and
medicine. Now intracellular effects by organelle EP are of growing interest, mainly due to …

Fundamentals of electroporative delivery of drugs and genes

E Neumann, S Kakorin, K Tœnsing - Bioelectrochemistry and bioenergetics, 1999 - Elsevier
Electrooptical and conductometrical relaxation methods have given a new insight in the
molecular mechanisms of the electroporative delivery of drug-like dyes and genes (DNA) to …

Electropermeabilization of mammalian cells to macromolecules: control by pulse duration

MP Rols, J Teissié - Biophysical journal, 1998 - cell.com
Membrane electropermeabilization to small molecules depends on several physical
parameters (pulse intensity, number, and duration). In agreement with a previous study …

Single-cell electroporationfor gene transfer in vivo

K Haas, WC Sin, A Javaherian, Z Li, HT Cline - Neuron, 2001 - cell.com
We report an electroporation technique for targeting gene transfer to individual cells in intact
tissue. Electrical stimulation through a micropipette filled with DNA or other macromolecules …

Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis

S Satkauskas, MF Bureau, M Puc, A Mahfoudi… - Molecular therapy, 2002 - cell.com
Efficient cell electrotransfection can be achieved using combinations of high-voltage (HV;
800 V/cm, 100 μs) and low-voltage (LV; 80 V/cm, 100 ms) pulses. We have developed …