Although they are often exploited to facilitate the expression and purification of recombinant
proteins, every affinity tag, whether large or small, has the potential to interfere with the …

Conjugation or deconjugation of ubiquitin (Ub) or ubiquitin‐like proteins (UBLs) to or from
cellular proteins is a multifaceted and universal means of regulating cellular physiology …

Marine bacterium Vibrio natriegensis a novel host platform for different applications in
molecular biology and biotechnology. It has one of the fastest growth rates of any known …

Fusion protein technologies improve the expression and purification of recombinant
proteins, but the removal of the tags involved requires specific proteases. The circularly …

Rapid and efficient tag removal remains a significant problem in recombinant protein
purification. Using an engineered DnaE intein from Nostoc punctiforme, we developed a …

The reversible conjugation of ubiquitin and ubiquitin-like (UbL) proteins to protein substrates
plays a critical role in the regulation of many cellular pathways. The removal of ubiquitin from …

A new peptide trypsin inhibitor named BWI-2c was obtained from buckwheat (Fagopyrum
esculentum) seeds by sequential affinity, ion exchange and reversed-phase …

Enteropeptidase is a serine protease used in different biotechnological applications. For
many applications the smaller light chain can be used to avoid the expression of the rather …

Caspase-2 is the most specific protease of all caspases and therefore highly suitable as tag
removal enzyme creating an authentic N-terminus of overexpressed tagged proteins of …

TRAIL (tumor necrosis factor (TNF) related apoptosis-inducing ligand) has been introduced
as an extrinsic pathway inducer of apoptosis that does not have the toxicities of Fas and …