Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Alternative to FBS in animal cell culture-An overview and future perspective

KS Chelladurai, JDS Christyraj, K Rajagopalan… - Heliyon, 2021 - cell.com
Fetal bovine serum (FBS) is a widely used growth supplement in the in vitro culturing of
animal and human cells, tissues and organs, notably due to the occurrence of abundant …

Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

[HTML][HTML] Quantitative approaches in multimodal fundus imaging: state of the art and future perspectives

A Arrigo, E Aragona, MB Parodi, F Bandello - Progress in Retinal and Eye …, 2023 - Elsevier
When it first appeared, multimodal fundus imaging revolutionized the diagnostic workup and
provided extremely useful new insights into the pathogenesis of fundus diseases. The recent …

Photoluminescent ZnO nanoparticles and their biological applications

ZY Zhang, HM Xiong - Materials, 2015 - mdpi.com
During the past decades, numerous achievements concerning luminescent zinc oxide
nanoparticles (ZnO NPs) have been reported due to their improved luminescence and good …

[HTML][HTML] Fluorescence lifetime imaging ophthalmoscopy

C Dysli, S Wolf, MY Berezin, L Sauer, M Hammer… - Progress in retinal and …, 2017 - Elsevier
Imaging techniques based on retinal autofluorescence have found broad applications in
ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus …

Designing a rigorous microscopy experiment: Validating methods and avoiding bias

APT Jost, JC Waters - Journal of Cell Biology, 2019 - rupress.org
Images generated by a microscope are never a perfect representation of the biological
specimen. Microscopes and specimen preparation methods are prone to error and can …

Opportunities for persistent luminescent nanoparticles in luminescence imaging of biological systems and photodynamic therapy

DL Fritzen, L Giordano, LCV Rodrigues… - Nanomaterials, 2020 - mdpi.com
The use of luminescence in biological systems allows us to diagnose diseases and
understand cellular processes. Persistent luminescent materials have emerged as an …

[HTML][HTML] Rapid diagnosis and intraoperative margin assessment of human lung cancer with fluorescence lifetime imaging microscopy

M Wang, F Tang, X Pan, L Yao, X Wang, Y Jing, J Ma… - BBA clinical, 2017 - Elsevier
A method of rapidly differentiating lung tumor from healthy tissue is extraordinarily needed
for both the diagnosis and the intraoperative margin assessment. We assessed the ability of …

[HTML][HTML] Impact of macular pigment on fundus autofluorescence lifetimes

L Sauer, D Schweitzer, L Ramm… - … & visual science, 2015 - jov.arvojournals.org
Purpose: To characterize the macular region and to investigate the influence of the macular
pigment (MP) on fundus autofluorescence (FAF) lifetimes in vivo. Methods: Forty-eight …