Capillary electrophoresis for quantitative studies of biomolecular interactions

VA Galievsky, AS Stasheuski, SN Krylov - Analytical chemistry, 2015 - ACS Publications
The development of highly selective and sensitive analytical techniques has been a driving
force for unprecedented advances in biotechnology, gene engineering, and drug discovery …

Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014)

BC Durney, CL Crihfield, LA Holland - Analytical and bioanalytical …, 2015 - Springer
This review of capillary electrophoresis methods for DNA analyses covers critical advances
from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone …

Non-SELEX isolation of DNA aptamers for the homogeneous-phase fluorescence anisotropy sensing of tau Proteins

S Lisi, E Fiore, S Scarano, E Pascale, Y Boehman… - Analytica chimica …, 2018 - Elsevier
Herein, we report for the first time the isolation of DNA aptamers directed against the whole
tau protein, an important Alzheimer's disease (AD) biomarker. Non-SELEX approach based …

Using nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) for simultaneous determination of concentration and equilibrium constant

M Kanoatov, VA Galievsky, SM Krylova… - Analytical …, 2015 - ACS Publications
Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) is a versatile
tool for studying affinity binding. Here we describe a NECEEM-based approach for …

Study of molecular interactions by nonequilibrium capillary electrophoresis of equilibrium mixtures: Originations, developments, and applications

L Deng, Q Fu, Y Zhang, F Shui, J Tang, J Wu… - …, 2023 - Wiley Online Library
Molecular interactions play a vital role in regulating various physiological and biochemical
processes in vivo. Kinetic capillary electrophoresis (KCE) is an analytical platform that offers …

Selection of bovine catalase aptamers using non‐SELEX

J Ashley, K Ji, SFY Li - Electrophoresis, 2012 - Wiley Online Library
In this research, we used the non‐SELEX method to successfully select an aptamer that
binds to the protein target (bovine catalase) with a KD value in the low micro molar range …

Unusual complexation behavior between daclatasvir and γ-Cyclodextrin. A multiplatform study

S Krait, A Salgado, C Villani, L Naumann… - … of chromatography A, 2020 - Elsevier
During a screening of cyclodextrins (CDs) as chiral selectors for the separation of daclatasvir
(DCV) and its enantiomer by capillary electrophoresis (CE), an unusual phenomenon for …

Ion mobility mass spectrometry enables the discrimination of positional isomers and the detection of conformers from cyclic oligosaccharides-metals supramolecular …

V Bonnet, G Clodic, C Sonnendecker… - Carbohydrate …, 2023 - Elsevier
Cyclic oligosaccharides are well known to interact with various metals, able to form
supramolecular complexes with distinct sizes and shapes. However, the presence of various …

Capillary electrophoresis‐based methodology for screening of oligonucleotide aptamers

X Guo, GH Chen - Biomedical Chromatography, 2021 - Wiley Online Library
As a new molecular recognition element, oligonucleotide aptamer not only has higher
affinity and specificity to target molecules, but also has the advantages of wide recognition …

Three-dimensional selection of leptin aptamers using capillary electrophoresis and implications for clone validation

J Ashley, SFY Li - Analytical biochemistry, 2013 - Elsevier
Capillary electrophoresis–systematic evolution of ligands by exponential enrichment (CE–
SELEX) has been used as a fast and efficient way to select aptamers against protein targets …