Hydrophilic interaction liquid chromatography (HILIC) has emerged as a valuable
complimentary technique to reversed-phase (RP), being especially suited for the analysis of …

Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …

Protein N-and O-glycosylation are well known co-and post-translational modifications of
immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence …

The pharmaceutical industry is one of the most regulated industries worldwide, since the
drug products have to be safe and effective. The levels of impurities and degradation …

N-glycosylation of therapeutic antibodies starts as a co-translational step followed by a set of
post-translational modifications and is considered as one of the critical quality attributes …

Increasing attention has been paid to developability assessment with the understanding that
thorough evaluation of monoclonal antibody lead candidates at an early stage can avoid …

Over the past few years, loss of patent protection for blockbuster monoclonal antibody (mAb)
drugs has caused a significant shift in the pharmaceutical industry towards the development …

Antibody–drug conjugates (ADCs) are biopharmaceuticals produced by chemically linking
small molecules (payloads) to antibodies that possess specific affinity for the target cell. The …

Among all the–omics approaches, proteomics and metabolomics have received increased
attention over the last decade. Both approaches have reached a certain level of maturity …

With an enormous growth in the application of hydrophilic interaction liquid chromatography
(HILIC), there has also been significant progress in HILIC method development. HILIC is a …