The burden of cardiovascular and metabolic diseases worldwide is staggering. The
emergence of systems approaches in biology promises new therapies, faster and cheaper …

Zebrafish (Danio rerio) has been a prominent model vertebrate in a variety of biological
disciplines. Substantial information gathered from developmental and genetic research …

CRISPR-Cas9 technology provides a powerful system for genome engineering. However,
variable activity across different single guide RNAs (sgRNAs) remains a significant …

In a large-scale screen, we isolated mutants displaying a specific visible phenotype in
embryos or early larvae of the zebrafish, Danio rerio. Males were mutagenized with …

Heart disease is the leading cause of death worldwide. Despite decades of research, most
heart pathologies have limited treatments, and often the only curative approach is heart …

The zebrafish (Danio rerio) is a powerful model organism for the study of vertebrate biology,
being well suited to both developmental and genetic analysis. Large-scale genetic screens …

We have used confocal microangiography to examine and describe the vascular anatomy of
the developing zebrafish, Danio rerio. This method and the profound optical clarity of …

The zinc finger transcription factor GATA4 has been implicated in heart development based
on its early expression in precardiogenic splanchnic mesoderm and its ability to activate the …

Zebrafish possess a unique yet poorly understood capacity for cardiac regeneration. Here,
we show that regeneration proceeds through two coordinated stages following resection of …

The appearance of molecular differences between arterial and venous endothelial cells
before circulation suggests that genetic factors determine these cell types. We find that …