Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

We have developed new open-source software called cis TEM (computational imaging
system for transmission electron microscopy) for the processing of data for high-resolution …

The bacterial flagellum is the prototypical protein nanomachine and comprises a rotating
helical propeller attached to a membrane-embedded motor complex. The motor consists of a …

Precise three-dimensional (3D) atomic structure determination of individual nanocrystals is a
prerequisite for understanding and predicting their physical properties. Nanocrystals from …

Single-molecule long-read DNA sequencing with biological nanopores is fast and high-
throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the …

The type 9 secretion system (T9SS) is the protein export pathway of bacteria of the Gram-
negative Fibrobacteres–Chlorobi–Bacteroidetes superphylum and is an essential …

Export of proteins through type III secretion systems is critical for motility and virulence of
many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) …

We present CryoGAN, a new paradigm for single-particle cryo-electron microscopy (cryo-
EM) reconstruction based on unsupervised deep adversarial learning. In single-particle cryo …

Three classes of ion-driven protein motors have been identified to date: ATP synthase, the
bacterial flagellar motor and a proton-driven motor that powers gliding motility and the type 9 …