Virus-induced gene silencing (VIGS): a powerful tool for crop improvement and its advancement towards epigenetics
S Zulfiqar, MA Farooq, T Zhao, PP Wang… - International journal of …, 2023 - mdpi.com
Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that
has evolved into an indispensable approach for analyzing the function of genes. It …
has evolved into an indispensable approach for analyzing the function of genes. It …
Breakthrough in CRISPR/Cas system: Current and future directions and challenges
Targeted genome editing (GE) technology has brought a significant revolution in fictional
genomic research and given hope to plant scientists to develop desirable varieties. This …
genomic research and given hope to plant scientists to develop desirable varieties. This …
Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes
Q Liu, C Zhao, K Sun, Y Deng, Z Li - Molecular Plant, 2023 - cell.com
CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …
biology research and crop breeding. However, the lack of robust delivery methods has …
Heritable gene editing in tomato through viral delivery of isopentenyl transferase and single-guide RNAs to latent axillary meristematic cells
D Liu, EE Ellison, EA Myers, LI Donahue… - Proceedings of the …, 2024 - pnas.org
Realizing the full potential of genome editing for crop improvement has been slow due to
inefficient methods for reagent delivery and the reliance on tissue culture for creating gene …
inefficient methods for reagent delivery and the reliance on tissue culture for creating gene …
Getting better all the time—recent progress in the development of CRISPR/Cas-based tools for plant genome engineering
N Capdeville, P Schindele, H Puchta - Current opinion in biotechnology, 2023 - Elsevier
Highlights●Adapting new nucleases and improving established ones expand the
CRISPR/Cas toolbox.●Optimized base editors enlarge editing windows and enable CG …
CRISPR/Cas toolbox.●Optimized base editors enlarge editing windows and enable CG …
Guide RNA library-based CRISPR screens in plants: opportunities and challenges
Highlights•Recent advances in CRISPR–Cas-mediated genetic screening are summarized.•
Compared to arrayed screens, pooled CRISPR screens are more accessible in …
Compared to arrayed screens, pooled CRISPR screens are more accessible in …
Development of virus-induced genome editing methods in Solanaceous crops
Genome editing (GE) using CRISPR/Cas systems has revolutionized plant mutagenesis.
However, conventional transgene-mediated GE methods have limitations due to the time …
However, conventional transgene-mediated GE methods have limitations due to the time …
Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
D Tuo, Y Yao, P Yan, X Chen, F Qu, W Xue, J Liu… - Plant Methods, 2023 - Springer
Background Plant virus vectors designed for virus-mediated protein overexpression (VOX),
virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost …
virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost …
[HTML][HTML] Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors
Viral vectors hold enormous potential for genome editing in plants as transient delivery
vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral …
vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral …
Viral vectors as carriers of genome-editing reagents
The presence of a transgene in the genome of plants is a regulatory challenge. Recently, Liu
et al. reported an engineered tomato spotted wilt virus (TSWV) that can carry large clustered …
et al. reported an engineered tomato spotted wilt virus (TSWV) that can carry large clustered …