Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …
insights into a broad spectrum of biological processes such as metabolic imaging, protein …
High-throughput, multi-parametric, and correlative fluorescence lifetime imaging
In this review, we discuss methods and advancements in fluorescence lifetime imaging
microscopy that permit measurements to be performed at faster speed and higher resolution …
microscopy that permit measurements to be performed at faster speed and higher resolution …
siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
M Raspe, KM Kedziora, B Van Den Broek, Q Zhao… - Nature …, 2016 - nature.com
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method
for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of …
for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of …
Nanoparticle-and microparticle-based luminescence imaging of chemical species and temperature in aquatic systems: a review
Most aquatic systems rely on a multitude of biogeochemical processes that are coupled with
each other in a complex and dynamic manner. To understand such processes, minimally …
each other in a complex and dynamic manner. To understand such processes, minimally …
Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy
Two-photon fluorescence lifetime imaging microscopy (FLIM) is a widely used technique in
biomedical optical imaging. Presently, many two-photon time-domain FLIM setups are …
biomedical optical imaging. Presently, many two-photon time-domain FLIM setups are …
[HTML][HTML] Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging
We report the characterisation of gated optical image intensifiers for fluorescence lifetime
imaging, evaluating the performance of several different prototypes that culminate in a new …
imaging, evaluating the performance of several different prototypes that culminate in a new …
FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope
JP Dumas, JY Jiang, EM Gates… - … of biomedical optics, 2019 - spiedigitallibrary.org
We demonstrate the possibility of measuring FRET efficiency with a low-cost frequency-
domain fluorescence lifetime imaging microscope (FD-FLIM). The system utilizes single …
domain fluorescence lifetime imaging microscope (FD-FLIM). The system utilizes single …
Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging
We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM)
method that surpasses current FLIM techniques in multiplexing capability. The method …
method that surpasses current FLIM techniques in multiplexing capability. The method …
Histogram clustering for rapid time-domain fluorescence lifetime image analysis
We propose a histogram clustering (HC) method to accelerate fluorescence lifetime imaging
(FLIM) analysis in pixel-wise and global fitting modes. The proposed method's principle was …
(FLIM) analysis in pixel-wise and global fitting modes. The proposed method's principle was …