Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

M Ahmad, M Hirz, H Pichler, H Schwab - Applied microbiology and …, 2014 - Springer
Pichia pastoris is an established protein expression host mainly applied for the production of
biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished …

[HTML][HTML] Cultivation strategies to enhance productivity of Pichia pastoris: a review

V Looser, B Bruhlmann, F Bumbak, C Stenger… - Biotechnology …, 2015 - Elsevier
Pichia pastoris, a methylotrophic yeast, is an established system for the production of
heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise …

Recombinant protein production in yeasts

D Mattanovich, P Branduardi, L Dato, B Gasser… - Recombinant gene …, 2012 - Springer
Recombinant protein production is a multibillion-dollar market. The development of a new
product begins with the choice of a production host. While one single perfect host for every …

Regulation of Pichia pastoris promoters and its consequences for protein production

T Vogl, A Glieder - New biotechnology, 2013 - Elsevier
The methylotrophic yeast Pichia pastoris is a widely used host for heterologous protein
production. Along with favorable properties such as growth to high cell density and high …

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

B Gasser, M Saloheimo, U Rinas, M Dragosits… - Microbial cell …, 2008 - Springer
Different species of microorganisms including yeasts, filamentous fungi and bacteria have
been used in the past 25 years for the controlled production of foreign proteins of scientific …

Materials of marine origin: a review on polymers and ceramics of biomedical interest

TH Silva, A Alves, BM Ferreira… - International …, 2012 - journals.sagepub.com
Marine organisms are constituted by materials with a vast range of properties and
characteristics that may justify their potential application within the biomedical field …

Over‐expression of secreted proteins from mammalian cell lines

AC Dalton, WA Barton - Protein science, 2014 - Wiley Online Library
Secreted mammalian proteins require the development of robust protein over‐expression
systems for crystallographic and biophysical studies of protein function. Due to complex …

Komagataella phaffii as Emerging Model Organism in Fundamental Research

L Bernauer, A Radkohl, LGK Lehmayer… - Frontiers in …, 2021 - frontiersin.org
Komagataella phaffii (Pichia pastoris) is one of the most extensively applied yeast species in
pharmaceutical and biotechnological industries, and, therefore, also called the biotech …

[HTML][HTML] Genome and metabolic engineering in non-conventional yeasts: current advances and applications

AK Löbs, C Schwartz, I Wheeldon - Synthetic and systems biotechnology, 2017 - Elsevier
Microbial production of chemicals and proteins from biomass-derived and waste sugar
streams is a rapidly growing area of research and development. While the model yeast …

[HTML][HTML] New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris

T Vogl, FS Hartner, A Glieder - Current opinion in biotechnology, 2013 - Elsevier
Biopharmaceuticals are an integral part of modern medicine and pharmacy. Both, the
development and the biotechnological production of biopharmaceuticals are highly cost …