Gene expression analysis using real-time PCR has become an integral part of biomedical
research. Appropriate data normalization based on stably expressed housekeeping genes …

The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a
technique for mRNA expression analysis in different tissues. Real-time PCR is widely used …

Reference genes are used as internal controls in gene expression studies, but their
expression levels vary according to tissue types and experimental treatments. Quantitative …

Keratinocytes provide the first line of defense of the human body against carcinogenic
ultraviolet (UV) radiation. Acute and chronic UVB-mediated cellular responses were widely …

Based on the exquisite sensitivity, reproducibility and wide dynamic range of quantitative
reverse‐transcription real‐time polymerase chain reaction (qRT‐PCR), it is currently the …

Chronic exposure of the skin to ultraviolet B (UVB) radiation induces oxidative stress, which
plays a crucial role in the induction of skin aging. In this study, potential protective effect of …

In the next-generation sequencing era, RT-qPCR is still widely employed to quantify levels
of nucleic acids of interest due to its popularity, versatility, and limited costs. The …

Background The knowledge about chronic spontaneous urticaria (CSU) phenotypes is
based on its clinical characteristics, associated comorbidities, course of the disease, and its …

Psoriasis is a chronic autoimmune skin disease driven by dysregulations at the cellular,
genomic and genetic levels. MicroRNAs are key mediators of gene expression regulation …

Data normalization of gene expression on human dermal fibroblasts (HDF) exposed to UVA
has commonly been done using either GAPDH or β-actin as reference genes without any …