Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Synthetic biology transforms the way we perceive biological systems. Emerging
technologies in this field affect many disciplines of science and engineering. Traditionally …

Arsenic methylation contributes to the formation and diversity of environmental
organoarsenicals, an important process in the arsenic biogeochemical cycle. The arsM gene …

Synthetic biology applications rely on a well-characterized set of microbial strains, with an
established toolbox of molecular biology methods for their genetic manipulation. Since there …

Pseudomonas putida is a soil bacterium with multiple uses in fermentation and
biotransformation processes. P. putida ATCC 12633 can biotransform benzaldehyde and …

Environmental isolates are promising candidates for new chassis of synthetic biology
because of their inherent capabilities, which include efficiently converting a wide range of …

Microbial degradation of petroleum hydrocarbons plays a vital role in mitigating petroleum
contamination and heavy oil extraction. In this study, a Saccharomyces cerevisiae capable …

The valorization of lignocellulosic biomass, derived from various bio-waste materials, has
received considerable attention as a sustainable approach to improve production chains …

Research on Cas9 nucleases from different organisms holds great promise for advancing
genome engineering and gene therapy tools, as it could provide novel structural insights …

Arsenic (As) pollution is a widespread problem worldwide. In recent years, biosensors
based on enzymatic inhibition have been developed for arsenic detection, making the study …