An NMR view of protein dynamics in health and disease
Biological molecules are often highly dynamic, and this flexibility can be critical for function.
The large range of sampled timescales and the fact that many of the conformers that are …
The large range of sampled timescales and the fact that many of the conformers that are …
From structure to function: Route to understanding lncRNA mechanism
J Graf, M Kretz - BioEssays, 2020 - Wiley Online Library
RNAs have emerged as a major target for diagnostics and therapeutics approaches.
Regulatory nonprotein‐coding RNAs (ncRNAs) in particular display remarkable versatility …
Regulatory nonprotein‐coding RNAs (ncRNAs) in particular display remarkable versatility …
Dynamic basis for dG• dT misincorporation via tautomerization and ionization
Abstract Tautomeric and anionic Watson–Crick-like mismatches have important roles in
replication and translation errors through mechanisms that are not fully understood. Here …
replication and translation errors through mechanisms that are not fully understood. Here …
m1A and m1G disrupt A-RNA structure through the intrinsic instability of Hoogsteen base pairs
The B-DNA double helix can dynamically accommodate GC and AT base pairs in either
Watson–Crick or Hoogsteen configurations. Here, we show that G-C+ (in which+ indicates …
Watson–Crick or Hoogsteen configurations. Here, we show that G-C+ (in which+ indicates …
Chemical exchange
AG Palmer III, H Koss - Methods in enzymology, 2019 - Elsevier
The phenomenon of chemical or conformational exchange in NMR spectroscopy has
enabled detailed characterization of time-dependent aspects of biomolecular function …
enabled detailed characterization of time-dependent aspects of biomolecular function …
Characterizing micro-to-millisecond chemical exchange in nucleic acids using off-resonance R1ρ relaxation dispersion
This review describes off-resonance R 1ρ relaxation dispersion NMR methods for
characterizing microsecond-to-millisecond chemical exchange in uniformly 13 C/15 N …
characterizing microsecond-to-millisecond chemical exchange in uniformly 13 C/15 N …
NMR Chemical Exchange Measurements Reveal That N6-Methyladenosine Slows RNA Annealing
H Shi, B Liu, F Nussbaumer… - Journal of the …, 2019 - ACS Publications
N 6-Methyladenosine (m6A) is an abundant epitranscriptomic modification that plays
important roles in many aspects of RNA metabolism. While m6A is thought to mainly function …
important roles in many aspects of RNA metabolism. While m6A is thought to mainly function …
Challenges and perspectives for structural biology of lncRNAs—the example of the Xist lncRNA A-repeats
Following the discovery of numerous long non-coding RNA (lncRNA) transcripts in the
human genome, their important roles in biology and human disease are emerging. Recent …
human genome, their important roles in biology and human disease are emerging. Recent …
Insights into Watson–Crick/Hoogsteen breathing dynamics and damage repair from the solution structure and dynamic ensemble of DNA duplexes containing m1A
In the canonical DNA double helix, Watson–Crick (WC) base pairs (bps) exist in dynamic
equilibrium with sparsely populated (∼ 0.02–0.4%) and short-lived (lifetimes∼ 0.2–2.5 ms) …
equilibrium with sparsely populated (∼ 0.02–0.4%) and short-lived (lifetimes∼ 0.2–2.5 ms) …
Aromatic Ring Dynamics, Thermal Activation, and Transient Conformations of a 468 kDa Enzyme by Specific 1H–13C Labeling and Fast Magic-Angle Spinning NMR
Aromatic residues are located at structurally important sites of many proteins. Probing their
interactions and dynamics can provide important functional insight but is challenging in …
interactions and dynamics can provide important functional insight but is challenging in …