Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste
material produced by heterologous overexpression of recombinant genes. In industrial …

Flexibility or rigidity of the linker between two fused proteins is an important parameter that
affects the function of fusion proteins. In this study, we constructed a linker library with five …

In industry, enzymes are often immobilized to generate more stable enzyme preparations
that are easier to store, handle, and recycle for repetitive use. Traditionally, enzymes are …

Bacterial inclusion bodies (IBs) consist of unfolded protein aggregates and represent
inactive waste products often accumulating during heterologous overexpression of …

Green fluorescent protein (GFP)-tagging is the prevalent strategy to monitor protein
dynamics in living cells. However, the consequences of appending the bulky GFP moiety to …

Background In the last few decades, several groups have observed that proteins expressed
as inclusion bodies (IBs) in bacteria could still be biologically active when terminally fused to …

When a defined protein/peptide (or combinations thereof) control and define the synthesis of
an inorganic nanoparticle, the result is a cloneable NanoParticle (cNP). This is because the …

In this paper, we propose a fluorescence-lifetime imaging microscopy (FLIM) multiplexing
system based on the fluorogen-activating protein FAST. This genetically encoded …

An increasing number of both prokaryotic and eukaryotic cell types are being adapted as
platforms for recombinant protein production. The overproduction of proteins in such …