CRISPR/Cas-based modifications for therapeutic applications: a review
N Bharathkumar, A Sunil, P Meera, S Aksah… - Molecular …, 2022 - Springer
The CRISPR–Cas genome editing system is an intrinsic property of a bacteria-based
immune system. This employs a guide RNA to detect and cleave the PAM-associated target …
immune system. This employs a guide RNA to detect and cleave the PAM-associated target …
[HTML][HTML] Anticipating and identifying collateral damage in genome editing
Genome editing has powerful applications in research, healthcare, and agriculture.
However, the range of possible molecular events resulting from genome editing has been …
However, the range of possible molecular events resulting from genome editing has been …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics
The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins.
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …
Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria
D Collias, E Vialetto, J Yu, K Co, ÉH Almási… - Nature …, 2023 - nature.com
Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to
counter-select against unedited cells. However, editing normally requires efficient …
counter-select against unedited cells. However, editing normally requires efficient …
Sniper2L is a high-fidelity Cas9 variant with high activity
Although several high-fidelity SpCas9 variants have been reported, it has been observed
that this increased specificity is associated with reduced on-target activity, limiting the …
that this increased specificity is associated with reduced on-target activity, limiting the …
Harnessing non-Watson–Crick's base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cells
Genomic DNA of the cyanophage S-2L virus is composed of 2-aminoadenine (Z), thymine
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …
Optimization of Cas9 activity through the addition of cytosine extensions to single-guide RNAs
M Kawamata, HI Suzuki, R Kimura… - Nature Biomedical …, 2023 - nature.com
The precise regulation of the activity of Cas9 is crucial for safe and efficient editing. Here we
show that the genome-editing activity of Cas9 can be constrained by the addition of cytosine …
show that the genome-editing activity of Cas9 can be constrained by the addition of cytosine …
Negative DNA supercoiling induces genome-wide Cas9 off-target activity
CRISPR-Cas9 is a powerful gene-editing technology; however, off-target activity remains an
important consideration for therapeutic applications. We have previously shown that force …
important consideration for therapeutic applications. We have previously shown that force …
High-fidelity, hyper-accurate, and evolved mutants rewire atomic-level communication in CRISPR-Cas9
The high-fidelity (HF1), hyper-accurate (Hypa), and evolved (Evo) variants of the CRISPR-
associated protein 9 (Cas9) endonuclease are critical tools to mitigate off-target effects in the …
associated protein 9 (Cas9) endonuclease are critical tools to mitigate off-target effects in the …