Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Metabolism has long been considered as a relatively stiff set of biochemical reactions. This
somewhat outdated and dogmatic view has been challenged over the last years, as multiple …

Porphyrins and their derivatives find extensive applications in medicine, food, energy and
materials. In this study, we produced porphyrin compounds by combining Rhodobacter …

Highlights•Terpenoids have substantial pharmaceutical and biotechnological
relevance.•Microbial biosynthesis of terpenoids is of economic and ecological significance.• …

Microbial cell factories are the workhorses of industrial biotechnology and improving their
performances can significantly optimize industrial bioprocesses. Microbial strain …

A highly effective metabolic pathway is the key for an efficient cell factory. However, the
engineered homologous or heterologous multi-gene pathway may be unbalanced …

The valorization of CO 2 into valuable products is a sustainable strategy to help overcome
the climate crisis. In particular, biological conversion is attractive as it can produce long …

Alphaproteobacteria have a variety of cellular and metabolic features that provide important
insights into biological systems and enable biotechnologies. For example, some species are …

Engineered microorganisms have emerged as viable alternatives for limonene production.
However, issues such as low enzyme abundance or activities, and regulatory …

The versatile photosynthetic α-proteobacterium Rhodobacter sphaeroides, has recently
been extensively engineered as a novel microbial cell factory (MCF) to produce …