Paramagnetic chemical probes for studying biological macromolecules
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …
The role of protein loops and linkers in conformational dynamics and allostery
Proteins are dynamic entities that undergo a plethora of conformational changes that may
take place on a wide range of time scales. These changes can be as small as the rotation of …
take place on a wide range of time scales. These changes can be as small as the rotation of …
Visualizing transient dark states by NMR spectroscopy
Myriad biological processes proceed through states that defy characterization by
conventional atomic-resolution structural biological methods. The invisibility of these …
conventional atomic-resolution structural biological methods. The invisibility of these …
Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca2+-ligated calmodulin
Recent advances in rapid mixing and freeze quenching have opened the path for time-
resolved electron paramagnetic resonance (EPR)-based double electron-electron …
resolved electron paramagnetic resonance (EPR)-based double electron-electron …
Dynamozones are the most obvious sign of the evolution of conformational dynamics in HIV-1 protease
M Rahimi, M Taghdir, F Abasi Joozdani - Scientific Reports, 2023 - nature.com
Proteins are not static but are flexible molecules that can adopt many different
conformations. The HIV-1 protease is an important target for the development of therapies to …
conformations. The HIV-1 protease is an important target for the development of therapies to …
Quantitative agreement between conformational substates of holo calcium-loaded calmodulin detected by double electron–electron resonance EPR and predicted by …
Calcium-loaded calmodulin (CaM/4Ca2+) comprises two domains that undergo rigid body
reorientation from a predominantly extended conformation to a compact one upon binding …
reorientation from a predominantly extended conformation to a compact one upon binding …
Practical aspects of paramagnetic relaxation enhancement in biological macromolecules
GM Clore - Methods in enzymology, 2015 - Elsevier
In this brief review, we summarize various aspects of NMR paramagnetic relaxation
enhancement (PRE). We discuss the types of spin labels used in NMR studies, describe the …
enhancement (PRE). We discuss the types of spin labels used in NMR studies, describe the …
Calmodulin: The switch button of calcium signaling
CF Yang, WC Tsai - Tzu Chi Medical Journal, 2022 - journals.lww.com
Calmodulin (CaM), a calcium sensor, decodes the critical calcium-dependent signals and
converts them into the driving force to control various important cellular functions, such as …
converts them into the driving force to control various important cellular functions, such as …
Allostery of multidomain proteins with disordered linkers
Highlights•An analytic equation is derived for the allosteric free energy change in
multidomain proteins with disordered linkers.•The role of disordered linker is characterized …
multidomain proteins with disordered linkers.•The role of disordered linker is characterized …
Interplay between conformational selection and induced fit in multidomain protein–ligand binding probed by paramagnetic relaxation enhancement
GM Clore - Biophysical chemistry, 2014 - Elsevier
The binding of ligands and substrates to proteins has been extensively studied for many
years and can be described, in its simplest form, by two limiting mechanisms: conformational …
years and can be described, in its simplest form, by two limiting mechanisms: conformational …