In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

This review summarizes the current state of methods and results achievable by cryo-electron
microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to …

Cryo-EM has emerged as the most important technique for structure determination of
macromolecular complexes. However, raw cryo-EM maps often exhibit loss of contrast at …

Advances in microscopy instruments and image processing algorithms have led to an
increasing number of cryo-electron microscopy (cryo-EM) maps. However, building accurate …

Plasma, the fourth and most pervasive state of matter in the visible universe, is a fascinating
medium that is connected to the beginning of our universe itself. Man-made plasmas are at …

Accumulation of filamentous aggregates of tau protein in the brain is a pathological hallmark
of Alzheimer's disease (AD) and many other neurodegenerative tauopathies. The filaments …

An increasing number of protein structures are being determined by cryogenic electron
microscopy (cryo-EM). Although the resolution of determined cryo-EM density maps is …

Adeno-associated virus (AAV) has a single-stranded DNA genome encapsidated in a small
icosahedrally symmetric protein shell with 60 subunits. AAV is the leading delivery vector in …

As a discipline, structural biology has been transformed by the three-dimensional electron
microscopy (3DEM)“Resolution Revolution” made possible by convergence of robust cryo …

Cryo-EM experiments produce images of macromolecular assemblies that are combined to
produce three-dimensional density maps. Typically, atomic models of the constituent …