Enzyme-linked immunosorbent assay: an adaptable methodology to study SARS-CoV-2 humoral and cellular immune responses

AI Portilho, G Gimenes Lima, E De Gaspari - Journal of Clinical Medicine, 2022 - mdpi.com
The Enzyme-Linked Immunosorbent Assay is a versatile technique, which can be used for
several applications. It has enormously contributed to the study of infectious diseases. This …

Reduced sensitivity of commercial spike-specific antibody assays after primary infection with the SARS-CoV-2 Omicron variant

DN Springer, T Perkmann, CM Jani… - Microbiology …, 2022 - Am Soc Microbiol
ABSTRACT The SARS-CoV-2 Omicron variant is characterized by substantial changes in
the antigenic structure of the Spike (S) protein. Therefore, antibodies induced by primary …

Comprehensive comparison of seven SARS-CoV-2-specific surrogate virus neutralization and anti-spike IgG antibody assays using a live-virus neutralization assay as …

M Graninger, CM Jani, E Reuberger… - Microbiology …, 2023 - Am Soc Microbiol
Neutralizing antibodies (nAbs) are considered a valuable marker for measuring humoral
immunity against SARS-CoV-2. However, live-virus neutralization tests (NTs) require high …

Evaluation of three quantitative anti-SARS-CoV-2 antibody immunoassays

S Chapuy-Regaud, M Miédougé… - Microbiology …, 2021 - Am Soc Microbiol
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December
2019 and caused a dramatic pandemic. Serological assays are used to check for …

Long-term monitoring of SARS-CoV-2 seroprevalence and variants in Ethiopia provides prediction for immunity and cross-immunity

S Merkt, S Ali, EK Gudina, W Adissu, A Gize… - Nature …, 2024 - nature.com
Under-reporting of COVID-19 and the limited information about circulating SARS-CoV-2
variants remain major challenges for many African countries. We analyzed SARS-CoV-2 …

[HTML][HTML] Performance evaluation of four surrogate Virus Neutralization Tests (sVNTs) in comparison to the in vivo gold standard test

M Pieri, M Infantino, M Manfredi, M Nuccetelli… - Frontiers in Bioscience …, 2022 - imrpress.com
Background: Several commercial surrogate Virus Neutralization Tests (sVNTs) have been
developed in the last year. Neutralizing anti-SARS-CoV-2 antibodies through interaction …

The serological sciences network (SeroNet) for COVID-19: depth and breadth of serology assays and plans for assay harmonization

AB Karger, JD Brien, JM Christen, S Dhakal, TJ Kemp… - MSphere, 2022 - Am Soc Microbiol
ABSTRACT In October 2020, the National Cancer Institute (NCI) Serological Sciences
Network (SeroNet) was established to study the immune response to COVID-19, and “to …

Assay harmonization study to measure immune response to SARS-CoV-2 infection and vaccines: a serology methods study

TJ Kemp, HA Hempel, Y Pan, D Roy… - Microbiology …, 2023 - Am Soc Microbiol
ABSTRACT The Coronavirus disease 2019 (COVID-19) pandemic presented the scientific
community with an immediate need for accurate severe acute respiratory syndrome …

Studying temporal titre evolution of commercial SARS-CoV-2 assays reveals significant shortcomings of using BAU standardization for comparison

I Kroidl, S Winter, R Rubio-Acero, A Bakuli… - Virology Journal, 2023 - Springer
Background Measuring specific anti-SARS-CoV-2 antibodies has become one of the main
epidemiological tools to survey the ongoing SARS-CoV-2 pandemic, but also vaccination …

Evaluation of two rapid lateral flow tests and two surrogate ELISAs for the detection of SARS-CoV-2 specific neutralizing antibodies

P Girl, K Zwirglmaier, H von Buttlar, R Wölfel… - Frontiers in …, 2022 - frontiersin.org
As vaccination against SARS-CoV-2 progresses rapidly around the world, reliable detection
of SARS-CoV-2 specific neutralizing antibodies (NAb) has become an indispensable …