Hololectin interdomain linker determines asparaginyl endopeptidase-mediated maturation of antifungal hevein-like peptides in oats

S Loo, SV Tay, A Kam, W Lee, JP Tam - Frontiers in plant science, 2022 - frontiersin.org
Heveins and hevein-containing (hev-) lectins play important roles in stress and pathogenic
responses in plants but cause health concerns in humans. Hev-hololectins contain multiple …

Substrate‐binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains

X Hemu, NY Chan, HT Liew, S Hu, X Zhang… - New …, 2023 - Wiley Online Library
Peptide asparaginyl ligases (PALs) are useful tools for precision modifications of proteins
and live‐cell surfaces by ligating peptides after Asn/Asp (Asx). They share high sequence …

Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation

X Hemu, X Zhang, HY Chang, JE Poh… - Journal of Biological …, 2023 - ASBMB
Plant legumains are Asn/Asp-specific endopeptidases that have diverse functions in plants.
Peptide asparaginyl ligases (PALs) are a special legumain subtype that primarily catalyze …

An efficient peptide ligase engineered from a bamboo asparaginyl endopeptidase

XB Wang, CH Zhang, T Zhang, HZ Li, YL Liu… - The FEBS …, 2024 - Wiley Online Library
In recent years, a few asparaginyl endopeptidases (AEPs) from certain higher plants have
been identified as efficient peptide ligases with wide applications in protein labeling and …

Development of a general bioluminescent activity assay for peptide ligases

CH Zhang, XX Shao, XB Wang, LL Shou… - The FEBS …, 2022 - Wiley Online Library
In recent years, some peptide ligases have been identified, such as bacterial sortases and
certain plant asparaginyl or prolyl endopeptidases. Peptide ligases have wide applications …

Consensus design and engineering of an efficient and high-yield Peptide Asparaginyl Ligase

X Hemu, X Zhang, HY Chang, PJ En, JP Tam - bioRxiv, 2022 - biorxiv.org
Abstract Plant legumains are Asn/Asp-specific endopeptidases (AEPs) that have diverse
functions in plants. Peptide asparaginyl ligases (PALs) are a special legumain subtype that …

Theranostics for Oncological Therapy: The Results of World Research and Development Methods

LA Romodin - Biophysics, 2023 - Springer
Theranostics is a field of medicine where drugs are developed that are both therapeutic
agents and tools for the early diagnosis of diseases. Their application is primarily associated …

Theranostics for oncological therapy: results from worldwide research and the paths of development

LA Romodin - Biofizika, 2023 - journals.rcsi.science
Theranostics is a field of medicine with the aim to establish tools for a specific targeted
therapy based on early diagnosis of diseases. This effect is primarily due to targeted delivery …