Chromatin, the template for epigenetic regulation, is a highly dynamic entity that is constantly
reshaped during early development and differentiation. Epigenetic modification of chromatin …

DNA methylation is a key layer of epigenetic regulation. The deposition of methylation marks
relies on the catalytic activity of DNA methyltransferases (DNMTs), and their active removal …

The CCCTC-binding factor (CTCF) works together with the cohesin complex to drive the
formation of chromatin loops and topologically associating domains, but its role in gene …

Parallel single-cell sequencing protocols represent powerful methods for investigating
regulatory relationships, including epigenome-transcriptome interactions. Here, we report a …

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and
transcriptome sequencing that allows for the discovery of associations between …

Single-cell multi-omics has recently emerged as a powerful technology by which different
layers of genomic output—and hence cell identity and function—can be recorded …

The role of cellular metabolism in regulating cell proliferation and differentiation remains
poorly understood. For example, most mammalian cells cannot proliferate without …

The molecular mechanisms and signalling pathways that regulate the in vitro preservation of
distinct pluripotent stem cell configurations, and their induction in somatic cells by direct …

We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to
accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells …

Current human pluripotent stem cells lack the transcription factor circuitry that governs the
ground state of mouse embryonic stem cells (ESC). Here, we report that short-term …